RUNX1 antibody (AA 6-10)

Catalog No. ABIN871507

This Rabbit Polyclonal antibody specifically detects RUNX1 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RUNX1 antibody (AA 6-10) (ABIN871507)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RUNX1 Antibody

Binding Specificity: AA 6-10

Specificity: The antibody detects endogenous level of total AML1 (RUNX1) protein.

Purification: Affinity purified

Immunogen: Peptide sequence around AA 6-10 (D-A-S-T-S) derived from Human AML1 (RUNX1) . Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: AML1 (RUNX1)

Background: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The a subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.

Molecular Weight: 55 kDa

Gene ID: 861

NCBI Accession: NP_001116

UniProt: Q01196