Topoisomerase I antibody (N-Term)

Catalog No. ABIN926651

The Rabbit Polyclonal anti-Topoisomerase I antibody is suitable to detect Topoisomerase I in samples from Human. It has been validated for WB.

Quick Overview for Topoisomerase I antibody (N-Term) (ABIN926651)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Topoisomerase I Antibody

Binding Specificity: N-Term

Purification: Purified

Immunogen: TOP1 antibody was raised in rabbit using the N terminal of TOP1 as the immunogen

Application Details

Application Notes: Optimal conditions should be determined by the investigator.

Comment:

TOP1 Blocking Peptide, (ABIN5616730), is also available for use as a blocking control in assays to test for specificity of this TOP1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: TOP1

Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. Synonyms: Polyclonal TOP1 antibody, Anti-TOP1 antibody, topoisomerase, DNA I antibody, TOPI antibody.

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance