GALE antibody (Middle Region)

Catalog No. ABIN928723

This Rabbit Polyclonal antibody specifically detects GALE in WB. It exhibits reactivity toward Human.

Quick Overview for GALE antibody (Middle Region) (ABIN928723)

Target: GALE (UDP-Galactose-4-Epimerase (GALE))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GALE antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GALE Antibody

Binding Specificity: Middle Region

Purification: Purified

Immunogen: GALE antibody was raised in rabbit using the middle region of GALE as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

GALE Blocking Peptide, (ABIN5613709), is also available for use as a blocking control in assays to test for specificity of this GALE antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for GALE

Target: GALE (UDP-Galactose-4-Epimerase (GALE))

Alternative Name: GALE

Background: GALE is an UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild to severe ('generalized' form). Synonyms: Polyclonal GALE antibody, Anti-GALE antibody, UDP-galactose-4-epimerase antibody.

Pathways: Response to Water Deprivation, Cellular Glucan Metabolic Process