NDC80 antibody

Catalog No. ABIN931089

The Mouse Monoclonal anti-NDC80 antibody (Clone 2497C3a) (ABIN931089) specifically detects NDC80 in WB, ICC and DB. The antibody is reactive with Human samples.

Quick Overview for NDC80 antibody (ABIN931089)

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Dot Blot (DB)

Clone: 2497C3a

Product Details anti-NDC80 Antibody

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: KNTC2 antibody was raised in mouse using recombinant Human Ndc80 Homolog, Kinetochore Complex Component (S.Cerevisiae) (Ndc80)

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: KNTC2 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: KNTC2

Background: HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved. Synonyms: Monoclonal KNTC2 antibody, Anti-KNTC2 antibody, Kinetochore associated 2 antibody, NDC80 antibody, HEC antibody, HEC1 antibody, TID3 antibody, hsNDC80 antibody.

Pathways: Maintenance of Protein Location