SKP2 antibody (AA 1-130)

Catalog No. ABIN931430

The Mouse Monoclonal anti-SKP2 antibody is suitable to detect SKP2 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for SKP2 antibody (AA 1-130) (ABIN931430)

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SKP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 6G9D10

Product Details anti-SKP2 Antibody

Binding Specificity: AA 1-130

Immunogen: SKP2 antibody was raised in Mouse using a purified recombinant fragment of SKP2(aa1-130) expressed in E. coli as the immunogen.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10,000, WB: 1:500-1:2000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Store at -20 °C for long term storage.

Target Details for SKP2

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Alternative Name: SKP2

Background: SKP2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs. SKP2 is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). Synonyms: Monoclonal SKP2 antibody, Anti-SKP2 antibody, SKP-2 antibody, SKP 2 antibody, S phase kinase associated protein 2 antibody, p45 antibody.

Pathways: Mitotic G1-G1/S Phases