RBCL (Isoform 1) antibody

Catalog No. ABIN93524

There are 3+ publications for this product available. The Chicken Polyclonal anti-RBCL antibody is suitable to detect RBCL in samples from Arabidopsis thaliana, Pea, Alfalfa, Spinach, Synechococcus sp., Ulmus, Thiobacillus, Spartina alterniflora, Potato, Pinus strobus, Microcystis aeruginosa, Lobaria pulmonaria and Diatom (Fragilariopsis). It has been validated for WB, EM, IF and IL.

Quick Overview for RBCL (Isoform 1) antibody (ABIN93524)

Target: RBCL

Reactivity: Arabidopsis thaliana, Pea, Alfalfa, Spinach, Synechococcus sp., Ulmus, Thiobacillus, Spartina alterniflora, Potato, Pinus strobus, Microcystis aeruginosa, Lobaria pulmonaria, Diatom (Fragilariopsis)

Host: Chicken

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Electron Microscopy (EM), Immunofluorescence (IF), Immunolocalization (IL)

Product Details

Binding Specificity: Isoform 1

Purpose: Anti-RbcL | Rubisco large subunit, form I (chicken)

Cross-Reactivity (Details): Arabidopsis thaliana, Fragilariopsis cylindrus, Lobaria pulmonaria, Medicago sativa, mixed phytoplankton, Microcystis aeruginosa PCC7806, Pinus strobus, Pisum sativum, Solanum tuberosum, Spartina alterniflora, Spinacia oleracea, Synechococcus sp. PCC7842, Thiobacillus sp. Ulmus sp.
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Algae,Dicots,Conifers,Liverworts,Mosses,Prochlorophytes,Welwitschia

Purification: Purified, total IgY

Immunogen: KLH-conjugated synthetic peptide derived from all known plant

Application Details

Application Notes: (IL) tested on a grass species, formaldehyde-fixed and paraffin-embedded tissue following the protocol from Gonzalez et al, (1998) Plant Physiol, V, 116, 1 : 10 000-1 : 20 000, 2 μg of total cellular protein, (WB)

Comment:

This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794).Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 16 µg/µL

Buffer: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: 4 °C

Storage Comment: Store at 4°C, make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Abdel-Ghany, Pilon: "MicroRNA-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15932-45, (2008) (PubMed).

Ensminger, Schmidt, Lloyd: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." in: The New phytologist, Vol. 177, Issue 2, pp. 428-42, (2008) (PubMed).

Menon, Dou, Heinhorst, Shively, Cannon: "Halothiobacillus neapolitanus carboxysomes sequester heterologous and chimeric RubisCO species." in: PLoS ONE, Vol. 3, Issue 10, pp. e3570, (2008) (PubMed).

Target Details

Target: RBCL

Background:

Background: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.

Additional information: Peptide target used to elicit this antibody is not conserved in type II Rubisco found in dinoflagellates and some photosynthetic bacteria

Molecular Weight: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)

Gene ID: 844754

UniProt: O03042, Q3ALL1