Rubisco large subunit (RbcL) (Isoform 1) antibody

Antigen: Rubisco large subunit (RbcL)

Binding Site: Isoform 1

Clonality: Polyclonal

Host: Chicken

Reactivity: Arabidopsis thaliana, Lobaria pulmonaria, Alfalfa (Medicago sativa), Phytoplankton, Pea (Pisum sativum), Potato (Solanum tuberosum), Smooth Cordgrass (Spartina alterniflora), Spinach (Spinacia oleracea), Synechococcus PCC 7842, Thiobacillus, Elm (Ulmus)

Application: Western Blotting (WB), Immunolocalization (IL)

Catalog no.: ABIN93524

Additional Information

Immunogen: KLH-conjugated synthetic peptide derived from all known plant,algal andcyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences,including Arabidopsis thaliana (AtCg00490), Sychococcus sp. Q3ALL1

Format: Liquid

Description: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes therate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundantproteins on Earth and its homology has been demonstrated from purple bacteriato flowering plants.

Specificity: Predicted reactivity: di and monocots, green algae, mosses, conifers, liverworts, welwitschia,prochlorophytes.

Application Details

Application Notes: Recommended Dilution 1: 10 000 - 1: 20 000 on 2 µg of total cellular protein, detected with standard ECL(WB), (IL) tested on a grass species, formaldehyde-fixed and paraffin-embeddedtissue following the protocol from Gonzalez et al. (1998) Plant Physiol. V. 116. Additional Information: peptide target used to elicit this antibody is not conserved in type II Rubisco foundin dinoflagellates and some photosynthetic bacteria. For those species product.

Concentration: 16 µg/µl

Buffer: total IgY in PBS pH 8.0+ 0.02% sodium azide

Storage: store at 4°C, make aliquots to avoid working with a stock. Please, Remember tospin tubes briefly prior to opening them to avoid any losses that might occur fromliquid material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Publications

Soares, Driscoll, Olmos et al.: "Adaxial/abaxial specification in the regulation of photosynthesis and stomatal opening with respect to light orientation and growth with CO2 enrichment in the C4 species Paspalum dilatatum." in: The New phytologist, Vol. 177, Issue 1, pp. 186-98, 2007 (PubMed).

Ensminger, Schmidt, Lloyd: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." in: The New phytologist, Vol. 177, Issue 2, pp. 428-42, 2008 (PubMed).

Abdel-Ghany, Pilon: "MicroRNA-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15932-45, 2008 (PubMed).

Menon, Dou, Heinhorst et al.: "Halothiobacillus neapolitanus carboxysomes sequester heterologous and chimeric RubisCO species." in: PLoS ONE, Vol. 3, Issue 10, pp. e3570, 2008 (PubMed).