ATP1B1 antibody (Subunit beta)

Catalog No. ABIN93533

There is 1 publication for this product available. The Chicken Polyclonal anti-ATP1B1 antibody is suitable to detect ATP1B1 in samples from Rat, Arabidopsis thaliana, Cow, Barley, Phaeodactylum tricornutum, Spinach, Synechocystis, Synechococcus sp. and Spartina alterniflora. It has been validated for WB, EM and IL.

Quick Overview for ATP1B1 antibody (Subunit beta) (ABIN93533)

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Reactivity: Rat, Arabidopsis thaliana, Cow, Barley, Phaeodactylum tricornutum, Spinach, Synechocystis, Synechococcus sp., Spartina alterniflora

Host: Chicken

Clonality: Polyclonal

Conjugate: This ATP1B1 antibody is un-conjugated

Application: Western Blotting (WB), Electron Microscopy (EM), Immunolocalization (IL)

Product Details anti-ATP1B1 Antibody

Binding Specificity: Subunit beta

Purpose: Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (chicken antibodies)

Cross-Reactivity (Details): Arabidopsis thaliana, Hordeum vulgare, Phaeodactylum tricornutum, Spartina alterniflora, Spinacia oleracea, Synechocystis PCC 6803, Synechococcus PCC 7942, beef muscle, Rat liver
Not reactive in: Archeal V-type ATP synthase

Predicted Reactivity: Acinetobacter baumannii,Bacillus subtilis,Brassica napus,Cannabis sativa,Clostridioides difficile,Cyanobacteria,E.coli K-12,Galdieria sulphuraria,Heliomicrobium modesticaldum Ice1,Manihot esculenta,Nicotiana plumbaginifolia,Saccharomyces cerevisiae,Salmonella typhimurium,Trichodesmium erythraeum,Triticum aestivum,Vitis vinifera,Zosteria marina,Yrsinia sp.

Purification: Purified, total IgY

Immunogen: KLH-conjugated synthetic peptide derived from available plant

Isotype: IgY

Application Details

Application Notes: 1 : 500 for localization of native enzyme by immunogold (IL), 1 : 5 000-1 : 8 000 (WB)

Comment:

results of immunogold studies using anti-AtpB antibody are published in Andersson et al. (2009)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: 4 °C

Storage Comment: Store at 2-8°C., make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-ATP1B1 antibody (ABIN93533)

Fuller, Craven, Hall, Kijek, Taft-Benz, Kawula: "RipA, a cytoplasmic membrane protein conserved among Francisella species, is required for intracellular survival." in: Infection and immunity, Vol. 76, Issue 11, pp. 4934-43, (2008) (PubMed).

Target Details for ATP1B1

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Alternative Name: AtpB

Background:

Background: ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.

Additional information: The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 ATP synthase and most known bacterial F-type ATP synthases, Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein

Molecular Weight: 53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)

Gene ID: 830768

UniProt: P19366, P83483, P06541, A8IQU3

Pathways: Thyroid Hormone Synthesis, Ribonucleoside Biosynthetic Process, SARS-CoV-2 Protein Interactome