Rubisco large subunit, form I and form II (RbcL) antibody

Antigen: Rubisco large subunit, form I and form II (RbcL)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii (C. reinhardtii), Cyanophora paradoxa, Emiliania huxleyi, Euglena gracilis, Gonyaulax polyedra, Guzmania hybrid, Heterosigma akashiwo, Micromonas pusilla, Moss (Physcomitrella patens), Red algae (Porphyra), Spinach (Spinacia oleracea), Synechococcus PCC 7942, Thalassiosira pseudonana, Prochlorococcus

Application: Western Blotting (WB), Tissue printing (TP), Immunofluorescence (IF), Confocal Microscopy (CM), Immunolocalization (IL)

Catalog no.: ABIN93539

Additional Information

Immunogen: KLH-conjugated synthetic peptide conserved across all known plant, algal and(cyano)bacterial RbcL protein sequences (form I L8S8 and form II L2), includingArabidopsis thaliana AtCg00490, Hordeum vulgare P05698, Oryza sativaP0C510, Chlamydomonas reinhardtii P00877, Synechococcus PCC 7920A5CKC5

Format: Lyophilized

Description: This antibody is especially suitable for quantifying of Rubisco in plant andalgal samples. Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes therate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrablyhomologous from purple bacteria to flowering plants and consists of two proteinsubunits, each present in 8 copies. In plants and green algae, the large subunit(~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa)is coded by a family of nuclear rbcS genes.

Specificity: Predicted reactivity: di and monocots, conifers, mosses, liverworts, welwitschia, green algae, red alge,brown algae, cryptomonad, cyanobacteria including prochlorophytes,gamma-proeobacteria, beta-proteobacteria, alpha proteobacteria.

Application Details

Application Notes: Recommended Dilution 1: 5000 - 10 000 with standard ECL (WB), 1: 800 (TP),immunofluorescence,confocal microscopy (IF), 1: 250 for images see Prins et al.(2008), detailed protocol available on request (IL). Additional Information: anti-RbcL can be used as a cellular [compartment marker] of plastid stroma(cytoplasm in cyanobacteria) and detects RbcL protein from 31.25 fmoles. As bothforms (I and II) are detected it is suitable for work with samples fromDinoflagellates, Haptophytes and Ochrophytes (diatoms, Raphidophytes, brownalgae) as well as higher plants. This antibody together with Agrisera Rubiscoprotein standard is very suitable to quantify Rubisco in plant and algalsamples.

Buffer: Affinity purified serum in PBS pH 7.4

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Publications

Publications: Masuda, Mizusawa, Narisawa et al.: "The bacterial stringent response, conserved in chloroplasts, controls plant fertilization." in: Plant & cell physiology, Vol. 49, Issue 2, pp. 135-41, 2008 (PubMed).

Prins, van Heerden, Olmos et al.: "Cysteine proteinases regulate chloroplast protein content and composition in tobacco leaves: a model for dynamic interactions with ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) vesicular bodies." in: Journal of experimental botany, Vol. 59, Issue 7, pp. 1935-50, 2008 (PubMed).

Keech, Pesquet, Gutierrez et al.: "Leaf senescence is accompanied by an early disruption of the microtubule network in Arabidopsis." in: Plant physiology, Vol. 154, Issue 4, pp. 1710-20, 2010 (PubMed). Method employed by authors: Western Blotting (WB) (1:5000).

Niewiadomska, Bilger, Gruca et al.: "CAM-related changes in chloroplastic metabolism of Mesembryanthemum crystallinum L." in: Planta, Vol. 233, Issue 2, pp. 275-85, 2011 (PubMed).

Zilliges, Kehr, Meissner et al.: "The cyanobacterial hepatotoxin microcystin binds to proteins and increases the fitness of microcystis under oxidative stress conditions." in: PLoS ONE, Vol. 6, Issue 3, pp. e17615, 2011 (PubMed). Method employed by authors: Western Blotting (WB) (1:50000).