Cytosolic Fructose-1,6-Bisphosphatase (Cfbpase) (Cfbpase) antibody

Antigen: Cytosolic Fructose-1,6-Bisphosphatase (Cfbpase) (Cfbpase)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Brassica Napus, Pinus sylvestris, Rice (Oryza sativa), Maize/Corn (Zea mays)

Application: Western Blotting (WB)

Catalog no.: ABIN93547

Additional Information

Alternative name: Cytosolic fructose-1,6-bisphosphatase (cFBPase)

Immunogen: overexpressed cytosolic fructose 1,6 bisphosphatase (cFBPase) derived from thesequence from Arabidopsis thaliana cFBPase Q9MA79

Format: Lyophilized

Description: Fructose-1,6 bisphosphatase (FBPase) (EC=3.1.311) is one of the regulatoryenzymes in the sucrose biosynthetic pathway. In non-photosynthetic tissues, itregulates the rate of gluconeogenesis. In photosynthetic tissues, two FBPaseisozymes (chloroplastic and cytosolic) play key roles in carbon assimilation andmetabolism. In photosynthetic tissues cFBPase (cytosolic fructose 1,6bisphosphatase) converts triose phosphates from the chloroplast to sucroseduring light hours. Alternative name: D-fructose-1,6-bisphosphate1-phosphohydrolase

Specificity: Predicted reactivity: dicots including Pisum sativum, Solanum tuberosum and monocots includingOryza sativa, Zea mays.

Application Details

Application Notes: Recommended Dilution 1: 5 000 with standard ECL (WB). Additional Information: Kinetic and allosteric properties of the plant cytosolic FBPase are remarkablysimilar to the mammalian and yeast FBPase, but differ greatly from those of thechloroplastic FBPase. The antibody could detect FBPase from the human COS-7cell line transfected with FBP1 expressing vector.

Purity: Serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Publications

Publications: Han, Chen, Dai et al.: "Isobaric tags for relative and absolute quantification- based comparative proteomics reveals the features of plasma membrane-associated proteomes of pollen grains and pollen tubes from Lilium davidii." in: Journal of integrative plant biology, Vol. 52, Issue 12, pp. 1043-58, 2010 (PubMed).

Ito, Batth, Petzold et al.: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." in: Journal of proteome research, 2010 (PubMed).