Cytochrome Oxidase Subunit II (COXII) antibody

Antigen: Cytochrome Oxidase Subunit II (COXII)

Synonyms: cydB, SCD78.13, qxtB

Clonality: Polyclonal

Host: Chicken

Reactivity: Arabidopsis thaliana, Cauliflower (Brassica oleracea), Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica

Application: Western Blotting (WB)

Catalog no.: ABIN93555

Additional Information

Immunogen: KLH-conugated synthetic peptide fully conserved in all available proteinsequences from eudicots including Arabidopsis thaliana AtmG00160, monocotsincluding Oryza sativa P04373 and Physcomitrella patens Q1XGA9

Format: Liquid

Description: Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in therespiratory chain in the inner mitochondrial membrane. Subunits 1-3 form thefunctional core of the enzyme complex. Subunit 2 (COXII) transfers the electronsfrom cytochrome c via its binuclear copper A center to the bimetallic center of thecatalytic subunit 1.

Specificity: Arabidopsis thaliana (leaf extract and isolated mitochondria). Predicted reactivity: eudicots, monocots, Physcomitrella patens.

Application Details

Application Notes: Recommended Dilution 1:1000 with standard ECL (WB).

Buffer: total IgY in PBS pH 8.0+ 0.02% sodium azide

Storage: store at 4°C, make aliquots to avoid working with a stock. Please, remember tospin tubes briefly prior to opening them to avoid any losses that might occur fromliquid material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Publications

Publications: Grant, Miller, Watling et al.: "Synchronicity of thermogenic activity, alternative pathway respiratory flux, AOX protein content, and carbohydrates in receptacle tissues of sacred lotus during floral development." in: Journal of experimental botany, Vol. 59, Issue 3, pp. 705-14, 2008 (PubMed).

Leroch, Neuhaus, Kirchberger et al.: "Identification of a novel adenine nucleotide transporter in the endoplasmic reticulum of Arabidopsis." in: The Plant cell, Vol. 20, Issue 2, pp. 438-51, 2008 (PubMed).

Moellering, Benning: "Phosphate regulation of lipid biosynthesis in Arabidopsis is independent of the mitochondrial outer membrane DGS1 complex." in: Plant physiology, Vol. 152, Issue 4, pp. 1951-9, 2010 (PubMed).

Yoshida, Watanabe, Hachiya et al.: "Distinct responses of the mitochondrial respiratory chain to long- and short-term high-light environments in Arabidopsis thaliana." in: Plant, cell & environment, 2011 (PubMed).