COXII antibody

Catalog No. ABIN334535

There are 4+ publications for this product available. The Rabbit Polyclonal anti-COXII antibody is suitable to detect COXII in samples from Cabbage, Physcomytrium Patens, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Arabidopsis thaliana, durum wheat, Zea mays, Vicia faba, Triticum aestivum, Rape, Quercus rubra, Picea abies, Nicotiana tabacum, Lilium longiflorum (Trumpet lily), Eriophorum vaginatum, Cucumis sativus, Cucumis melo, Chickpea, Betula nana, Barley and Actinidia chinensis. It has been validated for WB and BN PAGE.

Quick Overview for COXII antibody (ABIN334535)

Target: COXII (Cytochrome Oxidase Subunit II (COXII))

Reactivity: Cabbage, Physcomytrium Patens, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Arabidopsis thaliana, durum wheat, Zea mays, Vicia faba, Triticum aestivum, Rape, Quercus rubra, Picea abies, Nicotiana tabacum, Lilium longiflorum (Trumpet lily), Eriophorum vaginatum, Cucumis sativus, Cucumis melo, Chickpea, Betula nana, Barley, Actinidia chinensis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COXII antibody is un-conjugated

Application: Western Blotting (WB), Blue-native PAGE (BN PAGE)

Product Details anti-COXII Antibody

Purpose: Anti-COXII | Plant Cytochrome oxidase subunit II (affinity purified)

Specificity: Arabidopsis thaliana (leaf extract and isolated mitochondria).

Cross-Reactivity (Details): Actinidia chinensis, Arabidopsis thaliana (leaf extract and isolated mitochondria), Betula nana, Brassica napus, Brassica oleracea, Cicer aretinum, Cucumis melo, Cucumis sativus, Erophorum vaginatum, Hordeum vulgare, Lilium longifolorum, Nicotiana tabacum, Picea abies, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica, Physcomitrium patens, Triticum aestivum, Triticum durum Desf., Zea mays, Vicia faba, Quercus rubra
Not reactive in: Mangifera indica, Saccharina japonica

Predicted Reactivity: Cucumis melo,Glycine max,Gossypium hirsutum,Neurachne alopecuroidea,Neurachne minor,Neurachne muelleri,Oryza sativa,Physcomitrium patens,Pisum sativum,Triticum aestivum,Vigna radiata

Purification: Immunogen affinity purified serum

Immunogen: KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160

Application Details

Application Notes: 1 : 1000 (BN-PAGE), 1 : 1000 (WB)

Comment:

antibody detects COXII protein in total leaf extracts and isolated mitochondriaBlue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-COXII antibody (ABIN334535)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Wang, Vanlerberghe: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." in: Physiologia plantarum, (2013) (PubMed).

Hopff, Wienkoop, Lüthje: "The plasma membrane proteome of maize roots grown under low and high iron conditions." in: Journal of proteomics, Vol. 91, pp. 605-18, (2013) (PubMed).

Miller, Grant, Giles, Ribas-Carbo, Berry, Watling, Robinson: "In the heat of the night - alternative pathway respiration drives thermogenesis in Philodendron bipinnatifidum." in: The New phytologist, (2010) (PubMed).

Target Details for COXII

Target: COXII (Cytochrome Oxidase Subunit II (COXII))

Alternative Name: COXII

Background:

Background: Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2

Additional information: Cellular [compartment marker] of mitochondrial inner membrane

Molecular Weight: 29.4 | 30 kDa (for Arabidopsis thaliana)

Gene ID: 3989052

UniProt: P04373, Q1XGA9