ATP1B1 antibody (Subunit beta)

Catalog No. ABIN334537

This Rabbit Polyclonal antibody specifically detects ATP1B1 in WB, IF, BN PAGE and ExM. It exhibits reactivity toward Rat, Arabidopsis thaliana, Cow, Pig, Barley, Phaeodactylum tricornutum, Spinach, Seal, Pea, Plasmodium berghei, Poplar (Populus), Red Algae (Cyanidioschyzon), Robinia pseudoacacia, Salmon, Scaphoideus titanus, Selaginella martensii, Setaria viridis, Soybean, Tetrabaena socialis, Tomato, Toxoplasma gondii, Zea mays, Oryza sativa, Bacillus cereus, Barnyard Grass (Echinochloa crus-galli), Bryopsis corticulans, Camellia sinensis, Chicken, Chlamydomonas reinhardtii, Chlorella vulgaris, Chromochloris zofingiensis, Cotton, Dionaea muscipula, Galdieria sulphuraria, Ostreococcus tauri, Nicotiana tabacum, Nicotiana benthamiana, Neochloris oleoabundans, Nannochloropsis salina, Moniliophthora perniciosa, Migratory locust (Locusta migratoria), Helicobacter pylori and E. coli. It has been mentioned in 4+ publications

Quick Overview for ATP1B1 antibody (Subunit beta) (ABIN334537)

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Reactivity: Rat, Arabidopsis thaliana, Cow, Pig, Barley, Phaeodactylum tricornutum, Spinach, Seal, Pea, Plasmodium berghei, Poplar (Populus), Red Algae (Cyanidioschyzon), Robinia pseudoacacia, Salmon, Scaphoideus titanus, Selaginella martensii, Setaria viridis, Soybean, Tetrabaena socialis, Tomato, Toxoplasma gondii, Zea mays, Oryza sativa, Bacillus cereus, Barnyard Grass (Echinochloa crus-galli), Bryopsis corticulans, Camellia sinensis, Chicken, Chlamydomonas reinhardtii, Chlorella vulgaris, Chromochloris zofingiensis, Cotton, Dionaea muscipula, Galdieria sulphuraria, Ostreococcus tauri, Nicotiana tabacum, Nicotiana benthamiana, Neochloris oleoabundans, Nannochloropsis salina, Moniliophthora perniciosa, Migratory locust (Locusta migratoria), Helicobacter pylori, E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP1B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Blue-native PAGE (BN PAGE), Expansion Microscopy (ExM)

Product Details anti-ATP1B1 Antibody

Binding Specificity: Subunit beta

Purpose: Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit antibodies)

Specificity: The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 ATP synthase and most known bacterial F-type ATP synthases. Peptide used for antibody production is located ina beta sheet, which is partly exposed near the surface of the AtpB protein.

Cross-Reactivity (Details): Arabidopsis thaliana, Bacillus cereus, Bryopsis corticulans, Camelina sinensis, Chlamydomonas reinhardtii, Chlorella vulgaris, Chromochloris zofingiensis, Cyanidioschyzon merolae, Dionaea muscipula, Echinochloa crus/galli, Escherichia coli, Gossypium hirsutum, Helicobacter pylori, Hordeum vulgare, Gladieria sulphuraria, Glycine max, Lycopersicum esulentum, Moniliophthora perniciosa, Nannochloropsis salina, Neochloris oleoabundans (chlorophyta), Nicotiana bentamiana, Nicotiana tabacum, Oryza sp. (roots, leafs, pollen), Ostreococcus tauri, Pheodactylum tricornutum CCAP 1055/1, Pisum sativum, Plasmodium berghei, Populus sp., Robinia pseudoacacia, Scaphoideus titanus, Selaginella martensii, Setaria viridis, Solanum lycopersicum, Spinacia oleracea, Tetrabaena socialis, Toxoplasma gondii, Zea maysAnimal tissues from: cow, chicken, pig, rat, salmon, seal, Locusta migratoria
Not reactive in: Archeal V-type ATP synthase

Predicted Reactivity: Acinetobacter baumannii,Algae,Bacillus subtilis,Brassica napus,Cannabis sativa,Clostridioides difficile,Cyanobacteria,E.coli K-12,Eragrostis tef,Galdieria sulphuraria,Heliomicrobium modesticaldum Ice1,Manihot esculenta,Nicotiana plumbaginifolia,Saccharomyces cerevisiae,Salmonella typhimurium,Trichodesmium erythraeum,Triticum aestivum,Vitis vinifera,Zosteria marina,Yrsinia sp.

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide derived from available plant

Application Details

Application Notes: 1 : 100 (IF), 1 : 5000 (BN-PAGE), 1: 2500 (U-ExM), 1 : 2000-1 : 5 000 (WB)

Comment:

Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-ATP1B1 antibody (ABIN334537)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, (2011) (PubMed).

Berto, DAdamo, Bergantino, Vallese, Giacometti, Costantini: "The cyanobacterium Synechocystis sp. PCC 6803 is able to express an active [FeFe]-hydrogenase without additional maturation proteins." in: Biochemical and biophysical research communications, (2011) (PubMed).

Ahsan, Nakamura, Komatsu: "Differential responses of microsomal proteins and metabolites in two contrasting cadmium (Cd)-accumulating soybean cultivars under Cd stress." in: Amino acids, (2010) (PubMed).

Target Details for ATP1B1

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Alternative Name: AtpB

Background:

Background: ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.

Additional information: The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases. Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein.Anti-AtpB antibody was used as a loading control in Chlamydomonas reinhardtii and Synechocystis sp. PCC6803.This product can be sold containing proclin if requested

Molecular Weight: 53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)

Gene ID: 830768

UniProt: P19366, P83483, P06541, A8IQU3

Pathways: Thyroid Hormone Synthesis, Ribonucleoside Biosynthetic Process, SARS-CoV-2 Protein Interactome