»
Antibody
»
anti-Blood Group Lewis B
Blood Group Lewis B antibody
| Antigen |
|
| Clonality |
Monoclonal (2-25LE) |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA
|
| Catalog no. |
ABIN93925 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
234.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 3 to 5 Business days |
|
Immunogen
|
Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pureendocervical type according to the Fenoglio's classification).
|
|
Format
|
Purified
|
|
Isotype
|
IgG1 (Matching secondary antibodies)
|
|
Clone
|
2-25LE
|
|
Specificity
|
The antibody 2-25LE reacts with Lewis b blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelialcells, and in secretions of certain individuals.
|
|
Application Notes
|
Immunohistochemistry (paraffin sections) - Recommended dilution: 5 µg/ml. Positive control: colorectal adenocarcinoma. Application note: heat mediated antigen retrieval (sodium citrate), mAb incubation 1 hour /RT, detection DAB
|
|
Concentration
|
1 mg/ml
|
|
Purity
|
> 95% (by SDS-PAGE)
|
|
Purification
|
Purified from hybridoma culture supernatant by protein A-affinity chromatography.
|
|
Buffer
|
Phosphate buffered saline with 15 mM sodium azide, pH 7.4
|
|
Storage
|
Store at +4 o C for 6 months. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
|
|
Restrictions
|
For Research Use only
|
|
Publications
|
Creuzot-Garcher, Guerzider, Assem et al.: "Alteration of sialyl Lewis epitope expression in pterygium." in: Investigative ophthalmology & visual science, Vol. 40, Issue 8, pp. 1631-6, 1999 (PubMed).
|
Alternatives for antigen "Blood Group Lewis B", type "Antibodies"
