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Blood Group Lewis B antibody

Reactivity: Human, Guinea Pig, Pig IF, IHC (f), ISt Host: Mouse Monoclonal 2-25LE unconjugated
Catalog No. ABIN6153108
  • Target See all Blood Group Lewis B products
    Blood Group Lewis B
    Reactivity
    Human, Guinea Pig, Pig
    Host
    • 13
    Mouse
    Clonality
    • 13
    Monoclonal
    Conjugate
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Blood Group Lewis B antibody is un-conjugated
    Application
    • 11
    • 8
    • 8
    • 5
    • 2
    • 2
    • 1
    • 1
    Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)
    Purpose
    Mouse Monoclonal anti-Lewis-B (2-25LE), Purified, with BSA
    Characteristics
    The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Purification
    Purified
    Immunogen
    Mucin isolated from a human ovarian cyst fluid
    Clone
    2-25LE
    Isotype
    IgG1 kappa
  • Application Notes
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    Colon

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    200 μg/mL
    Buffer
    PBS/0.05 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Target
    Blood Group Lewis B
    Alternative Name
    Lewis-B (Blood Group Lewis B Products)
    Synonyms
    MUC5 antibody, TBM antibody, leB antibody, mucin 5AC, oligomeric mucus/gel-forming antibody, MUC5AC antibody
    Molecular Weight
    45 kDa
    Gene ID
    2525, 169238
    UniProt
    P21217
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