CD14 Molecule (CD14) antibody

Antigen: CD14 Molecule (CD14)

Synonyms: CD14

Clonality: Monoclonal (MEM-18)

Host: Mouse

Reactivity: Human, Primates

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func), ELISA, Radioimmunoassay (RIA)

Catalog no.: ABIN93963

Additional Information

Alternative name: CD14

Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from theurine of a patient suffering from proteinuria.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: MEM-18

Description: CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface ofmature monocytes, macrophages, and neutrophils, where serves as a multifunctionallipopolysaccharide receptor, it is also released to the serum both as a secreted andenzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in areaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serumprotein. The soluble sCD14 is able to discriminate slight structural differences betweenlipopolysaccharides and is important for neutralization of serum allochthonouslipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic,inflammatory and infectious processes.

Specificity: The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI(glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes,macrophages and weakly on granulocytes, also expressed by most tissue macrophages. Inhuman, the epitope recognized by MEM-18 is located between amino acids 57-64.HLDA III, WS Code M 253HLDA IV, WS Code M 314HLDA V, WS Code M MA087HLDA VI, WS Code M MA95

Application Details

Application Notes: Western Blotting. Application note: Non-reducing conditions. ELISA: The antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody B-A8 (cat. no. 11-304-C100). Functional Application: The antibody MEM-18 completely blocks binding of fluorescein (FITC) labeled bacterial LPSto the monocyte surface and it also blocks the binding of CD14 to the extracellular TLR2domain.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by precipitation methods.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Stem Cells, Innate Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Immunology, Inflammation

Restrictions: For Research Use only

Images

Western Blotting analysis (non-reducing conditions) of over-expressed human CD14 using anti-CD14 (MEM-18). Lane 1: whole cell lysate HEK 293 transfected with empty vector. Lane 2: tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA. Lane 3: whole cell lysate of HEK 293 transfected with human CD14 cDNA

Publications

Publications: Stöckl, Majdic, Fischer et al.: "Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2724-33, 2001 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Angel, Lala, Chen et al.: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." in: International immunology, Vol. 19, Issue 11, pp. 1271-9, 2007 (PubMed).

Weiss, Lichtenauer, Kirchner et al.: "Hepatic progenitor cells from adult human livers for cell transplantation." in: Gut, Vol. 57, Issue 8, pp. 1129-38, 2008 (PubMed).

von Schlieffen, Oskolkova, Schabbauer et al.: "Multi-hit inhibition of circulating and cell-associated components of the toll-like receptor 4 pathway by oxidized phospholipids." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 29, Issue 3, pp. 356-62, 2009 (PubMed).

Jursik, Prchal, Grillari-Voglauer et al.: "Large-scale production and characterization of novel CD4+ cytotoxic T cells with broad tumor specificity for immunotherapy." in: Molecular cancer research : MCR, Vol. 7, Issue 3, pp. 339-53, 2009 (PubMed).

Wu, Zhang, Zhang et al.: "Expansion of CD14+CD16+ peripheral monocytes among patients with aseptic loosening." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 58, Issue 9, pp. 561-70, 2009 (PubMed).

Svensson: "Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology." in: Methods in molecular biology (Clifton, N.J.), Vol. 531, pp. 187-202, 2009 (PubMed).

Kuronuma, Mitsuzawa, Takeda et al.: "Anionic pulmonary surfactant phospholipids inhibit inflammatory responses from alveolar macrophages and U937 cells by binding the lipopolysaccharide-interacting proteins CD14 and MD-2." in: The Journal of biological chemistry, Vol. 284, Issue 38, pp. 25488-500, 2009 (PubMed).

Miko?ajczyk, Skrzeczy?ska-Moncznik, Zarebski et al.: "Interaction of human peripheral blood monocytes with apoptotic polymorphonuclear cells." in: Immunology, Vol. 128, Issue 1, pp. 103-13, 2009 (PubMed).

Schrauf, Kirchberger, Majdic et al.: "The ssRNA genome of human rhinovirus induces a type I IFN response but fails to induce maturation in human monocyte-derived dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 7, pp. 4440-8, 2009 (PubMed).

Schwartzkopff, Grimm, Lankford et al.: "Platelet factor 4 (CXCL4) facilitates human macrophage infection with HIV-1 and potentiates virus replication." in: Innate immunity, 2009 (PubMed).