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Integrin beta 2 antibody (ITGB2)

Details for Product anti-ITGB2 Antibody No. ABIN94010, Supplier: Login to see New
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Synonyms ITGB2, 2E6, AI528527, Cd18, LAD, LCAMB, Lfa1, MF17, CD18, LFA-1, MAC-1, MFI7, AI256028, LTbetaR, Ltar, TNF-R-III, TNFCR, TNFR-RP, TNFR2-RP, TNFRrp, Tnfbr, Tnfrsf3, D12S370, LT-BETA-R, TNFR3, TNFRSF3
(427), (214), (111), (26), (24), (7), (6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
(302), (186), (155), (5), (2), (2)
Clonality (Clone)
Monoclonal ()
This Integrin beta 2 antibody is un-conjugated
(100), (75), (55), (19), (9), (7), (6), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(426), (241), (196), (107), (84), (83), (62), (54), (40), (35), (17), (11), (7), (6), (4), (3), (2), (2), (1), (1), (1)
Pubmed 8 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 4 to 5 Business Days
Immunogen Peripheral blood mononuclear cells
Clone MEM-148
Isotype IgG1
Specificity The antibody MEM-148 recognizes an epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 (integrin beta2 subunit, beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.HLDA VI, WS Code AS A052
No Cross-Reactivity Pig (Porcine)
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name ITGB2 (ITGB2 Antibody Abstract)
Background CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Pathways NF-kappaB Signaling
Application Notes Flow Cytometry: Recommended dilution: 8 µg/mL The antibody MEM-148 is excellent marker of activated myeloid cells.
Western Blotting:
Application note: Non-reducing conditions.
Functional Application: The antibody MEM-148 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Western Blotting (WB) image for anti-Integrin beta 2 antibody (ITGB2) (ABIN94010) Western Blotting analysis of PMA-activated neutrophils (Fig. A) and monocytes (Fig. B...
Western Blotting (WB) image for anti-Integrin beta 2 antibody (ITGB2) (ABIN94010) Western Blotting analysis of PMA-activated neutrophils (Fig. A) and monocytes (Fig. B...
Product cited in: Aubert, Yoon, Sloan et al.: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).

Drbal, Angelisová, Cerný et al.: "A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins." in: Immunobiology, Vol. 203, Issue 4, pp. 687-98, 2001 (PubMed).

Drbal, Angelisová, Cerný et al.: "Human leukocytes contain a large pool of free forms of CD18." in: Biochemical and biophysical research communications, Vol. 275, Issue 2, pp. 295-9, 2000 (PubMed).

Background publications Dassanayake, Maheswaran, Srikumaran: "Monomeric expression of bovine beta2-integrin subunits reveals their role in Mannheimia haemolytica leukotoxin-induced biological effects." in: Infection and immunity, Vol. 75, Issue 10, pp. 5004-10, 2007 (PubMed).

Peters, Sindrilaru, Wang et al.: "CD18 in monogenic and polygenic inflammatory processes of the skin." in: The journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research, Vol. 11, Issue 1, pp. 7-15, 2006 (PubMed).

Solovjov, Pluskota, Plow: "Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2." in: The Journal of biological chemistry, Vol. 280, Issue 2, pp. 1336-45, 2005 (PubMed).

Shang, Issekutz: "Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers." in: European journal of immunology, Vol. 28, Issue 6, pp. 1970-9, 1998 (PubMed).

Gao, Issekutz: "Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers." in: Immunology, Vol. 88, Issue 3, pp. 463-70, 1996 (PubMed).

Catalog No. ABIN94010
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