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Integrin beta 2 (ITGB2) antibody

Details for Product No. ABIN94010
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Antigen
Synonyms ITGB2, 2E6, AI528527, Cd18, LAD, LCAMB, Lfa1, MF17, CD18, LFA-1, MAC-1, MFI7, AI256028, LTbetaR, Ltar, TNF-R-III, TNFCR, TNFR-RP, TNFR2-RP, TNFRrp, Tnfbr, Tnfrsf3, D12S370, LT-BETA-R, TNFR3, TNFRSF3
Reactivity
Human
(256), (165), (98), (61), (53), (46), (36), (24), (1)
Host
Mouse
(179), (114), (102), (2), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(55), (40), (29), (14), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Application
Functional Studies (Func), Immunoprecipitation (IP), Flow Cytometry (FACS), Western Blotting (WB)
(260), (130), (113), (62), (45), (41), (40), (37), (34), (27), (15), (6), (3), (3), (2)
Pubmed 1 reference available
Quantity 0.1 mg
Options
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Catalog No. ABIN94010
319.44 $
Plus shipping costs $45.00

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Immunogen Peripheral blood mononuclear cells
Clone MEM-148
Isotype IgG1
Specificity The antibody MEM-148 recognizes an epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 (integrin beta2 subunit, beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.HLDA VI, WS Code AS A052
No Cross-Reactivity Pig (Porcine)
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name ITGB2
Background CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Application Notes Flow Cytometry: Recommended dilution: 8 µg/mL The antibody MEM-148 is excellent marker of activated myeloid cells.
Western Blotting:
Application note: Non-reducing conditions.
Functional Application: The antibody MEM-148 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
General Aubert, Yoon, Sloan et al.: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).

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