CD2 Molecule (CD2) antibody (Biotin)

Details for Product No. ABIN94022
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Antigen
Synonyms CD2, LFA-2, Ly-37, Ly37, SRBC, T11, CD2R, LFA2, OX-34, OX34
Reactivity
Human
(249), (108), (58), (15), (12), (10), (9), (7), (3), (1)
Host
Mouse
(264), (79), (62)
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
(55), (46), (36), (14), (10), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS)
(277), (72), (68), (68), (58), (37), (22), (20), (13), (12), (10), (9), (4), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN94022
363.00 $
Plus shipping costs $45.00

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  • +1 404 474 4654
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Immunogen Human peripheral T cells.
Clone MEM-65
Isotype IgG1
Specificity The antibody MEM-65 recognizes an unique epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T-lymphocytes and NK cells, also expressed by all thymocytes.HLDA VI, WS Code T 6T-012
Characteristics The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Purification Purified from ascites by protein-G affinity chromatography.
Alternative Name CD2
Background CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working dilution is 1: 1000. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
General Ilangumaran, Briol, Hoessli: "CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes." in: Blood, Vol. 91, Issue 10, pp. 3901-8, 1998 (PubMed).

Espagnolle, Depoil, Zaru et al.: "CD2 and TCR synergize for the activation of phospholipase Cgamma1/calcium pathway at the immunological synapse." in: International immunology, Vol. 19, Issue 3, pp. 239-48, 2007 (PubMed).

Muhammad, Schiller, Forster et al.: "Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 12, pp. 7672-80, 2009 (PubMed).

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