CD34 (CD34) antibody

Antigen: CD34 (CD34)

Clonality: Monoclonal (4H11[APG])

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN94077

Additional Information

Alternative name: CD34

Immunogen: Permanent human cell line derived from peripheral leucocytes of a patient suffering fromchronic myeloid leukaemia.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 4H11[APG]

Description: CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present onmany stem cell populations. It is a well established stem cell marker, though its expressionon human hematopoietic stem cells is reversible. CD34 probably serves as a surfacereceptor that undergoes receptor-mediated endocytosis and regulates adhesion,differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34expression is likely to represent a specific state of hematopoietic development that mayhave altered adhering properties with expanding and differentiating capabilities in both invitro and in vivo conditions.

Specificity: The antibody 4H11[APG] reacts with Class III epitope on CD34 (Mucosialin), a 110-115 kDamonomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitorscells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. Theantibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class IIIantibodies BIRMA K3 and 8G12 on KG1a cell line.HLDA VI, WS Code M MA58

Application Details

Application Notes: Western Blotting - Recommended dilution: 2 µg/ml. Positive control: Kg-1a human leukemia cell lysate. Negative control: JURKAT human leukemia T-cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml. Positive tissue: placenta endothelium

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by precipitation methods.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Hematopoietic Progenitors, Hematopoietic Stem Cells, Cardiogenesis, Lineage Markers, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC. Total viable cells were used for analysis.

Publications

Publications: Elknerovua, Lacinovua, Soucek et al.: "Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines." in: Neoplasma, Vol. 54, Issue 4, pp. 311-20, 2007 (PubMed).

Hernandez Mir, Helin, Skarp et al.: "Glycoforms of human endothelial CD34 that bind L-selectin carry sulfated sialyl Lewis x capped O- and N-glycans." in: Blood, Vol. 114, Issue 3, pp. 733-41, 2009 (PubMed).