CD34 (CD34) antibody (Biotin)
| Antigen |
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| Clonality |
Monoclonal (4H11[APG]) |
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Host
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Reactivity
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Human (177), Mouse (Murine) (86), Rat (Rattus) (33), Dog (Canine) (27), Cow (Bovine) (15), Pig (Porcine) (15), Rabbit (15), Monkey (6), Primate (5), Primates (3), Rhesus Monkey (2)
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Conjugate
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FITC (34), PE (25), Biotin (15), APC (8), RPE (6), Alexa Fluor 488 (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5,PE (2), Cy5.5 (2), Cy7 (2), Cy7,PE (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1), Cy5.5,PE (1), PE,DY647 (1), PE-Dyomics 647 (1), Pacific Blue (1)
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| Catalog no. |
ABIN94078 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
325.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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CD34
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Immunogen
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Permanent human cell line derived from peripheral leucocytes of a patient suffering fromchronic myeloid leukaemia.
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Isotype
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IgG1
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Clone
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4H11[APG]
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Description
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CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present onmany stem cell populations. It is a well established stem cell marker, though its expressionon human hematopoietic stem cells is reversible. CD34 probably serves as a surfacereceptor that undergoes receptor-mediated endocytosis and regulates adhesion,differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34expression is likely to represent a specific state of hematopoietic development that mayhave altered adhering properties with expanding and differentiating capabilities in both invitro and in vivo conditions.
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Specificity
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The antibody 4H11[APG] reacts with Class III epitope on CD34 (Mucosialin), a 110-115 kDamonomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitorscells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. Theantibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class IIIantibodies BIRMA K3 and 8G12 on KG1a cell line.HLDA VI, WS Code M MA58
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Application Notes
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Biotinylated antibody is designed for indirect immunofluorescence analysis by FlowCytometry. Suggested working dilution is 1: 200.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
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Concentration
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1 mg/ml
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Hematopoietic Progenitors, Hematopoietic Stem Cells, Cardiogenesis, Lineage Markers, CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Publications
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Elknerovua, Lacinovua, Soucek et al.: "Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines." in: Neoplasma, Vol. 54, Issue 4, pp. 311-20, 2007 (PubMed).
Hernandez Mir, Helin, Skarp et al.: "Glycoforms of human endothelial CD34 that bind L-selectin carry sulfated sialyl Lewis x capped O- and N-glycans." in: Blood, Vol. 114, Issue 3, pp. 733-41, 2009 (PubMed).
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Alternatives for antigen "CD34 (CD34)", type "Antibodies"
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Hosts
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Mouse (139), Rabbit (55), Rat (51), Chicken (1)
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Reactivities
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Human (177), Mouse (Murine) (86), Rat (Rattus) (33), Dog (Canine) (27), Cow (Bovine) (15), Pig (Porcine) (15), Rabbit (15), Monkey (6), Primate (5), Primates (3), Rhesus Monkey (2)
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Applications
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Flow Cytometry (FACS) (187), Western Blotting (WB) (86), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (54), Immunofluorescence (IF) (53), Immunoprecipitation (IP) (42), Immunohistochemistry (Frozen Sections) (IHC (fro)) (37), ELISA (28), Immunohistochemistry (IHC) (13), Immunocytochemistry (ICC) (11), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Functional Studies (Func) (3), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1)
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Conjugates
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FITC (34), PE (25), Biotin (15), APC (8), RPE (6), Alexa Fluor 488 (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5,PE (2), Cy5.5 (2), Cy7 (2), Cy7,PE (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1), Cy5.5,PE (1), PE,DY647 (1), PE-Dyomics 647 (1), Pacific Blue (1)
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Epitopes
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pTyr330 (1)
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Alternatives 
