CD44 (CD44) antibody

Antigen: CD44 (CD44)

Synonyms: IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CDW44, CSPG8, HCELL, MUTCH-I, ECMR-III, MGC10468

Clonality: Monoclonal (MEM-85)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), ELISA

Catalog no.: ABIN94123

Additional Information

Alternative name: CD44

Immunogen: Leukocytes of a patient suffering from LGL Type Leukaemia.

Format: Purified

Isotype: IgG2b  (Matching secondary antibodies)

Clone: MEM-85

Description: CD44 is a transmembrane glycoprotein expressed on the surface of most cells, whichserves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion,proliferation and migration, it is thus important for lymphocyte activation, recirculation andhoming, it can thus serve e.g. as a modulator of macrophage recruitment in response topathogen. Although CD44 functions are essential for physiological activities of normal cells,elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitatingtumour growth and metastasis, antiapoptosis and directional motility of cancer cells.

Specificity: The antibody MEM-85 reacts with both cell surface-expressed and soluble form of CD44antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherinfamily) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymalcells, etc.), it is negative on platelets and hepatocytes.HLDA IV, WS Code NL 706HLDA VI, WS Code AS Ref.15

Application Details

Application Notes: Western Blotting - Recommended dilution: 2 µg/ml, 60 min on vertical incubator Positive control: Kg-1a human leukemia cell lysate. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (6% separating gel).

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Cancer, Cell Cycle

Restrictions: For Research Use only

Publications

Publications: Ilangumaran, Briol, Hoessli: "CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes." in: Blood, Vol. 91, Issue 10, pp. 3901-8, 1998 (PubMed).