CD44 (CD44) antibody (Biotin)

Antigen: CD44 (CD44)

Synonyms: IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CDW44, CSPG8, HCELL, MUTCH-I, ECMR-III, MGC10468

Clonality: Monoclonal (MEM-85)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Catalog no.: ABIN94124

Additional Information

Alternative name: CD44

Immunogen: Leukocytes of a patient suffering from LGL Type Leukaemia.

Isotype: IgG2b

Clone: MEM-85

Description: CD44 is a transmembrane glycoprotein expressed on the surface of most cells, whichserves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion,proliferation and migration, it is thus important for lymphocyte activation, recirculation andhoming, it can thus serve e.g. as a modulator of macrophage recruitment in response topathogen. Although CD44 functions are essential for physiological activities of normal cells,elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitatingtumour growth and metastasis, antiapoptosis and directional motility of cancer cells.

Specificity: The antibody MEM-85 reacts with both cell surface-expressed and soluble form of CD44antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherinfamily) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymalcells, etc.), it is negative on platelets and hepatocytes.HLDA IV, WS Code NL 706HLDA VI, WS Code AS Ref.15

Application Details

Application Notes: Biotinylated antibody is designed for indirect immunofluorescence analysis by FlowCytometry. Suggested working dilution is 1: 1000.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Concentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Cancer, Cell Cycle

Restrictions: For Research Use only

Publications

Publications: Ilangumaran, Briol, Hoessli: "CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes." in: Blood, Vol. 91, Issue 10, pp. 3901-8, 1998 (PubMed).