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CD56 antibody (Neural Cell Adhesion Molecule 1) (Biotin)

Details for Product anti-NCAM1 Antibody No. ABIN94188, Supplier: Login to see
Antigen
  • NCAM1
  • ncam
  • n-cam
  • CD56
  • MSK39
  • NCAM
  • E-NCAM
  • NCAM-1
  • Ncam
  • Cd56
  • N-CAM
  • N-CAM-1
  • NCAM-C
  • NCAMC
  • fj56g09
  • ncam1
  • wu:fj56g09
Reactivity
Human, Non-Human Primate
615
96
73
15
8
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5
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3
3
3
3
2
1
Host
Mouse
528
105
16
11
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD56 antibody is conjugated to Biotin
60
53
38
27
9
8
8
8
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6
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
350
249
124
119
98
79
78
61
27
22
7
6
5
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Immunogen KG-1 human acute myelogenous leukemia cell line
Clone MEM-188
Isotype IgG2a
Specificity The antibody MEM-188 reacts with a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody MEM-188 could react with rhesus monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested. HLDA VI, WS code A055HLDA VI, WS Code NK26HLDA VII, WS code 70077
Characteristics The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Purification Purified from ascites by protein-A affinity chromatography.
Alternative Name NCAM1 (NCAM1 Antibody Abstract)
Background CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.
Pathways
Application Notes Working concentrations should be determined by the investigator.I t is recommended that the user titrates the reagent for use in the particular testing system.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-CD56 antibody (Neural Cell Adhesion Molecule 1)  (Biotin) (ABIN94188) Surface staining of human peripheral blood cells with anti-CD56 (MEM-188) FITC.
Product cited in: Hovden, Karlsen, Jonsson et al.: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, 2011 (PubMed).

Olsen, Tollefsen, Aagaard et al.: "Isolation of Mycobacterium avium subspecies paratuberculosis reactive CD4 T cells from intestinal biopsies of Crohn's disease patients." in: PLoS ONE, Vol. 4, Issue 5, pp. e5641, 2009 (PubMed).

Yates, Rovis, Mitchell et al.: "The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro." in: International immunology, Vol. 19, Issue 6, pp. 785-99, 2007 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Brdicka, Imrich, Angelisová et al.: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).

Background publications Jakovcevski, Mo, Zecevic: "Down-regulation of the axonal polysialic acid-neural cell adhesion molecule expression coincides with the onset of myelination in the human fetal forebrain." in: Neuroscience, Vol. 149, Issue 2, pp. 328-37, 2007 (PubMed).

Ohishi, Kaku, Oya et al.: "CD56 expression in ovarian granulosa cell tumors, and its diagnostic utility and pitfalls." in: Gynecologic oncology, Vol. 107, Issue 1, pp. 30-8, 2007 (PubMed).

McCluggage, McKenna, McBride: "CD56 is a sensitive and diagnostically useful immunohistochemical marker of ovarian sex cord-stromal tumors." in: International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists, Vol. 26, Issue 3, pp. 322-7, 2007 (PubMed).

Lin, Liu, Chen et al.: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005 (PubMed).

Lanier, Chang, Azuma et al.: "Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 12, pp. 4421-6, 1991 (PubMed).