Neural Cell Adhesion Molecule 1 (NCAM1) antibody (Biotin)

Antigen: Neural Cell Adhesion Molecule 1 (NCAM1)

Synonyms: CD56, NCAM, MSK39, ncam, fj56g09, wu:fj56g09, NCAM1, n-cam, LOC397761

Clonality: Monoclonal (MEM-188)

Host: Mouse

Reactivity: Human, Primate

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Catalog no.: ABIN94188

Additional Information

Alternative name: CD56

Immunogen: KG-1 human acute myelogenous leukemia cell line

Isotype: IgG2a

Clone: MEM-188

Description: CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein ofimmunoglobulin family serving as adhesive molecule which is ubiquitously expressed innervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on Tcells and NK cells. Polysialic modification results in reduction of CD56-mediated celladhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity.CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrinetumours and ovarian granulosa cell tumours.

Specificity: The antibody MEM-188 reacts with a 180 kDa isoform of CD56 (NCAM) expressed inleukocytes. It has been suggested that the antibody MEM-188 could react with rhesusmonkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.HLDA VI, WS code A055HLDA VI, WS Code NK26HLDA VII, WS code 70077

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis. Suggested working concentration is 5 µg/ml. Indicated dilution is recommended startingpoint for use of this product. Working concentrations should be determined by the investigator.

Concentration: 1 mg/ml

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: CD Antigens, Surface Receptors of Immune Cells, Signaling

Restrictions: For Research Use only

Publications

Brdicka, Imrich, Angelisovua et al.: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).

Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Lin, Liu, Chen et al.: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Olsen, Tollefsen, Aagaard et al.: "Isolation of Mycobacterium avium subspecies paratuberculosis reactive CD4 T cells from intestinal biopsies of Crohn's disease patients." in: PLoS ONE, Vol. 4, Issue 5, pp. e5641, 2009 (PubMed).