CD56 antibody (Internal Region)

Catalog No. ABIN768541

This Goat Polyclonal antibody specifically detects CD56 in FACS, WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for CD56 antibody (Internal Region) (ABIN768541)

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD56 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-CD56 Antibody

Binding Specificity: Internal Region

Purpose: CD56 / NCAM1 (isoform 2)

Sequence: KASWTRPEKQ ETLD

Specificity: This antibody is expected to recognize reported isoform 2 (NP_851996.2) only.

Cross-Reactivity: Cow, Human, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KASWTRPEKQETLD, from the internal region of the protein sequence according to NP_851996.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5 μg/mL.

Western Blot: Approx 150-170 kDa band observed in Human Cerebellum and Frontal Cortex lysates (calculated MW of 94.6 kDa according to NP_851996.2). The observed molecular weight corresponds to the glycosylated form and is routinely observed by other source

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Alternative Name: NCAM1

Background: NCAM1, neural cell adhesion molecule 1, CD56, MSK39, NCAM, N-CAM-1, NCAM-1, antigen recognized by monoclonal antibody 5.1H11, neural cell adhesion molecule, NCAM

Molecular Weight: 94.6kDa according to NP_851996.2

Gene ID: 4684, 24586

NCBI Accession: NP_851996