CD95 antibody
| Antigen |
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| Clonality |
Monoclonal (LT95) |
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Host
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Reactivity
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Application
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 Alternatives Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Flow Cytometry (FACS) (63), Western Blotting (WB) (34), Immunoprecipitation (IP) (26), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Functional Studies (Func) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Blocking Antibody (Inhibition) (8), ELISA (6), Immunofluorescence (IF) (5), ELISA (Detection) (3), Dot Blot (Dot) (2), Immunohistochemistry (IHC) (2)
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| Catalog no. |
ABIN94248 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
260.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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CD95 / Fas
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Immunogen
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HUT-78 human T cell lymphoma cell line
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Format
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Purified
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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LT95
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Description
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CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of theTNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular deathdomains, formation of the death-inducing signaling complex (DISC) and activation ofcaspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated atthe DISC, in type II cells low concentration of caspase 8 activates pathway leading to therelease of cytochrome c from mitochondria and activation of caspase 3 by cytochom c.Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokineresponses.
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Specificity
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The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoproteinof the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily,expressed on a variety of normal and neoplastic cells.It seems that the antibody LT95 does not induce Fas mediated apoptosis, although itcross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
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Application Notes
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Flow Cytometry: excellent Immunohistochemistry (paraffin sections) - Positive tissue: tonsil
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from ascites by precipitation methods and ion exchange chromatography.
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Buffer
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20 mM Tris/HCl with 15 mM sodium azide, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Restrictions
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For Research Use only
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Publications
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Drosopoulos, Roberts, Cermak et al.: "Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 22856-67, 2005 (PubMed).
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Alternatives for antigen "CD95", type "Antibodies"
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Hosts
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Mouse (91), Rabbit (20), Goat (1)
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Reactivities
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Human (109), Monkey (3), Mouse (Murine) (3), Rat (Rattus) (3)
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Applications
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Flow Cytometry (FACS) (63), Western Blotting (WB) (34), Immunoprecipitation (IP) (26), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Functional Studies (Func) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Blocking Antibody (Inhibition) (8), ELISA (6), Immunofluorescence (IF) (5), ELISA (Detection) (3), Dot Blot (Dot) (2), Immunohistochemistry (IHC) (2)
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Conjugates
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FITC (10), PE (10), APC (6), Biotin (4), SPRD (2)
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Epitopes
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C-Term (4), pTyr232 (2), pTyr291 (2), Center (1)
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