C-Src tyrosine Kinase (CSK) antibody

Antigen: C-Src tyrosine Kinase (CSK)

Synonyms: MGC117393, AW212630, MGC112926

Clonality: Monoclonal (CSK-04)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN94268

Additional Information

Alternative name: Csk

Immunogen: Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: CSK-04

Description: C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resemblesSrc-family kinases, but unlike them lacks the conserved autophosphorylation site, theregulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negativelyregulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine.Disruption of the csk gene causes constitutive activation of Src-family kinases, andoverexpression of Csk usually counteracts their signaling. The Csk-mediated regulation ofthose Src-family kinases that are localized in lipid rafts is enabled by a ubiquitouslyexpressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), whichrecruits Csk.

Specificity: The antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa non-receptorprotein tyrosine kinase expressed in cytosol.

Application Details

Application Notes: Western Blotting. Application note: The recommended dilution of the antibody CSK-04 is 1: 250-1: 300.Weak non-specific bandof cca 60 kDa is detected in human cell lines in lower antibody dilutions. Immunoprecipitation. Application note: The immunosorbent (CSK-04 antibody covalently bound to beads) immunoprecipitates Cskfrom cell lysates. Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Signaling, Tyrosine Kinases

Restrictions: For Research Use only

Images

Immunofluorescence staining of Csk in human HeLa cell line using anti-Csk (CSK-04, green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).