»
Antibody
»
anti-Keratin 7-17 KRT7-17
Keratin 7/17 (KRT7/17) antibody
| Antigen |
|
| Synonyms |
K7, CK7, SCL, K2C7, MGC3625, MGC129731, Krt2-7, MGC11625, D15Wsu77e, KRT7, MGC137771 |
| Clonality |
Monoclonal (C-46) |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
|
| Catalog no. |
ABIN94296 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
260.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 3 to 5 Business days |
|
Alternative name
|
Cytokeratin 7+17
|
|
Immunogen
|
Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
|
|
Format
|
Purified
|
|
Isotype
|
IgG1 (Matching secondary antibodies)
|
|
Clone
|
C-46
|
|
Description
|
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkablebiochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20different polypeptides, molecular weight between 40 kDa and 68 kDa. The individualcytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidiccytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
|
|
Specificity
|
The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratinsare a member of intermediate filaments subfamily represented in epithelial tissues. This antibody does not react with: Mouse, Rat, Rabbit.
|
|
Application Notes
|
Western Blotting - Recommended dilution: 1 µg/ml. Positive control: HeLa human cell line. Sample preparation: Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication, 5 min. in hotLaemmli buffer (reducing conditions. ) - Immunohistochemistry (paraffin sections) - Recommended dilution: 20 µg/ml Pretreatment: 0,1% pepsin, 30 min, 37 o C Incubation: 4 o C overnight Positive tissue: pancreas. Application note: chromogen DAB
|
|
Concentration
|
1 mg/ml
|
|
Purity
|
> 95% (by SDS-PAGE)
|
|
Purification
|
Purified from hybridoma culture supernatant by protein A-affinity chromatography.
|
|
Buffer
|
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
|
|
Storage
|
Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
|
|
Research Area
|
Extracellular Matrix
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "Keratin 7/17 (KRT7/17)", type "Antibodies"
