Keratin 7/17 antibody

Catalog No. ABIN7865681

This Mouse Monoclonal antibody specifically detects Keratin 7/17 in IHC, WB, ICC, FACS and IF. It exhibits reactivity toward Human, Pig, Dog and Sheep.

Quick Overview for Keratin 7/17 antibody (ABIN7865681)

Target: Keratin 7/17 (KRT7/17)

Reactivity: Human, Pig, Dog, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 7/17 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: C-46

Product Details anti-Keratin 7/17 Antibody

Purpose: anti-Keratin 7+17 mouse monoclonal, C-46, purified

Cross-Reactivity (Details): not with ferret, mouse, rabbit, rat

Purification: affinity chromatography

Immunogen: purified human keratin 7, isolated from HeLa cytoskeleton

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment and/ or pepsin digestion recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for Keratin 7/17

Target: Keratin 7/17 (KRT7/17)

Alternative Name: Keratin 7+17

Background: C-46 reacts with keratins 7 (54 kDa) and 17 (46 kDa). On formalin-fixed paraffin embedded sections C46 reacts with keratin 7 only. Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia. Positive control: Relevant simple epithelia like ovary, lung, and breast.