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HLA Class I Histocompatibility Antigen, alpha Chain E (HLAE) (Heavy Chain) antibody

Details for Product No. ABIN94352
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Antigen
Synonyms EA1.2, EA2.1, HLA-6.2, MHC, QA1
Epitope
Heavy Chain
(7), (4), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Non-Human Primate
(84), (4), (2), (1), (1), (1), (1)
Host
Mouse
(55), (29)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(45), (39), (32), (26), (10), (8), (4), (4), (1), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN94352
348.48 $
Plus shipping costs $45.00

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Immunogen Recombinant human HLA-E denaturated heavy chain.
Clone MEM-E-02
Isotype IgG1
Specificity The antibody MEM-E/02 specifically reacts with denaturated heavy chain of human HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of all human cell types.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name HLA-E
Research Area Major Histocompatibility Complex (MHC), Immunology
Application Notes Immunohistochemistry (paraffin sections) Recommended dilution: 5-10 µg/mL
Positive control: Human placenta
Application note: Heat antigen retrieval (sodium citrate),
Incubation with mAb: 1 hour / RT, detection DAB

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Product cited in: Menier, Saez, Horejsi et al.: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).

Dambaeva, Bondarenko, Grendell et al.: "Non-classical MHC-E (Mamu-E) expression in the rhesus monkey placenta." in: Placenta, Vol. 29, Issue 1, pp. 58-70, 2008 (PubMed).

Platonova, Cherfils-Vicini, Damotte et al.: "Profound coordinated alterations of intratumoral NK cell phenotype and function in lung carcinoma." in: Cancer research, Vol. 71, Issue 16, pp. 5412-22, 2011 (PubMed).

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