HLA-E antibody (HLA Class I Histocompatibility Antigen, alpha Chain E) (Biotin) Primary Antibody
- Target See all HLA-E Antibodies
- This HLA-E antibody is conjugated to Biotin
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells.
The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
- Cross-Reactivity (Details)
- Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
anti-HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E) (AA 251-358) antibody Primary Antibody
HLA-E Reactivity: Human ELISA, FACS, IF (cc), IF (p), IHC (fro), IHC (p), WB Host: Rabbit Polyclonal unconjugated
- Application Notes
- Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 2 microgram/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
- For Research Use only
- 1 mg/mL
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
Do not freeze.
Avoid prolonged exposure to light.
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
MicroRNA editing facilitates immune elimination of HCMV infected cells." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003963, (2014) (PubMed).
: "Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells." in: Neoplasia (New York, N.Y.), Vol. 13, Issue 9, pp. 822-30, (2011) (PubMed).
- MicroRNA editing facilitates immune elimination of HCMV infected cells." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003963, (2014) (PubMed).
- Alternative Name
- HLA-E (HLA-E Products)
- EA1.2, EA2.1, HLA-6.2, MHC, QA1, major histocompatibility complex, class I, E, HLA-E, PATR-E
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response