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HLA-E antibody (HLA Class I Histocompatibility Antigen, alpha Chain E) (Biotin) Primary Antibody

HLA-E Reactivity: Human FACS, IHC (p), IP Host: Mouse Monoclonal MEM-E-07 Biotin
Pubmed (2 references)
Catalog No. ABIN1302714
$551.03
Plus shipping costs $45.00
0.1 mg
Shipping to: United States
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  • Target See all HLA-E Antibodies
    HLA-E
    Reactivity
    • 75
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 52
    • 24
    Mouse
    Clonality
    • 54
    • 22
    Monoclonal
    Conjugate
    • 32
    • 8
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This HLA-E antibody is conjugated to Biotin
    Application
    • 40
    • 27
    • 27
    • 13
    • 13
    • 10
    • 9
    • 9
    • 3
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
    Specificity
    The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells.
    The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
    Cross-Reactivity (Details)
    Human
    Immunogen
    Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
    Clone
    MEM-E-07
    Isotype
    IgG1
  • Application Notes
    Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 2 microgram/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
    Comment

    The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
  • Nachmani, Zimmermann, Oiknine Djian, Weisblum, Livneh, Khanh Le, Galun, Horejsi, Isakov, Shomron, Wolf, Hengel, Mandelboim: "MicroRNA editing facilitates immune elimination of HCMV infected cells." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003963, (2014) (PubMed).

    Lo Monaco, Tremante, Cerboni, Melucci, Sibilio, Zingoni, Nicotra, Natali, Giacomini: "Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells." in: Neoplasia (New York, N.Y.), Vol. 13, Issue 9, pp. 822-30, (2011) (PubMed).

  • Target
    HLA-E
    Alternative Name
    HLA-E (HLA-E Products)
    Synonyms
    EA1.2, EA2.1, HLA-6.2, MHC, QA1, major histocompatibility complex, class I, E, HLA-E, PATR-E
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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