Kinesin (0) (Heavy chain) antibody

Antigen: Kinesin (0)

Synonyms: DK, DKH, DmKHC, Dmkin, KHC, KIF5A, KIN, kinesin, l(2)W12, l(2)k13219, l(2)k13314, l(2R)W12, DmelCG7765, CG7765, DKLC, KLC, DmelCG5433, CG5433

Epitope: Heavy chain

Clonality: Monoclonal (KN-03)

Host: Mouse

Reactivity: Human, Pig (Porcine), Mouse (Murine), Rat (Rattus)

Application: Immunocytochemistry (ICC)

Catalog no.: ABIN94413

Additional Information

Alternative name: Kinesin (heavy chain)

Immunogen: Enriched fraction of porcine brain kinesin.

Format: Purified

Isotype: IgM  (Matching secondary antibodies)

Clone: KN-03

Description: Kinesin belongs to the group of microtubule-associated motor proteins known to convertchemical energy released from nucleoside triphosphates (preferentially from ATP) intomechanical energy. Conventional kinesin, member of the kinesin superfamily comprisingmore than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells.Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of twoheavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular massabout 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both amicrotubule-binding site and an ATPase active center, stalk region responsible for heavychain dimerization and finally C-terminal globular tail domain, which is implicated in cargobinding. Light chains may have a regulatory function.

Specificity: The antibody KN-03 recognizes heavy chain of conventional kinesin associated withvesicles and with lower affinity with denaturated molecule. It stains Western blots ofkinesin-enriched preparations.Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed byimmunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with differentsets of fragments.The antibody KN-03 well recognizes kinesin bound to taxol-stabilized microtubules.

Application Details

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by thiophilic adsorption-affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. Do not freeze.

Research Area: Cytoskeleton

Restrictions: For Research Use only

Images

Immunofluorescence staining (rat basophils)

Publications

Publications: Mac?rek, Draberova, Richterova et al.: "Monoclonal antibodies KN-02 and KN-03 against the heavy chain of kinesin." in: Hybridoma and hybridomics, Vol. 21, Issue 6, pp. 457-62, 2003 (PubMed).