Kinesin (heavy chain) antibody

Details for Product No. ABIN94412
Antigen
Reactivity
Human, Pig (Porcine), Mouse (Murine), Rat (Rattus)
4
3
2
1
1
1
1
1
1
Host
Mouse
4
3
Clonality (Clone)
Monoclonal ()
Application
Immunocytochemistry (ICC)
6
5
4
3
2
2
1
1
Options
Immunogen Enriched fraction of porcine brain kinesin.
Clone KN-02
Isotype IgM
Specificity The antibody KN-02 recognizes heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations.
Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments.
The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.
Cross-Reactivity (Details) Mouse, Human, Rat, Other not tested, Porcine
Purification Purified by precipitation and chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Background Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.
Pathways Peptide Hormone Metabolism
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Kinesin (heavy chain) antibody (ABIN94412) Immunofluorescence staining (rat basophils) Immunofluorescence staining of vesicles (...
Product cited in: Malcová-Janatová, Richterová, Dráber, Hasek: "Preparation of human recombinant kinesin heavy chain and epitope mapping of its structural domains." in: Folia microbiologica, Vol. 49, Issue 6, pp. 665-70, 2005 (PubMed).

Mac?rek, Dráberová, Richterová, Böhm, Dráber: "Monoclonal antibodies KN-02 and KN-03 against the heavy chain of kinesin." in: Hybridoma and hybridomics, Vol. 21, Issue 6, pp. 457-62, 2003 (PubMed).