LIME antibody

Antigen: LIME

Clonality: Monoclonal (LIME-10)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN94426

Additional Information

Immunogen: COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated tokeyhole limpet hemocyanin.

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: LIME-10

Description: LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusuallybasic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cellcoreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fynkinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylationof C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase ofenzymatic activity compared with the total pool of Lck, thus, LIME serves as a positiveregulator of TCR-dependent T cell signaling. However, under some circumstances, LIMEmay mediate inhibitory signals.

Specificity: The antibody LIME-10 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interactingtransmembrane adaptor expressed by T cells.

Application Details

Application Notes: Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Immunology, Adaptive Immunity, CD Antigens

Restrictions: For Research Use only

Images

Western Blotting