V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) antibody

Antigen: V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN)

Synonyms: Yes, c-yes, HsT441, P61-YES, yes, LYN, YES1, hst441, p61-yes, MGC89200, JTK8, FLJ26625, Hck-2, AA407514, lyn

Clonality: Monoclonal (LYN-01)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN94427

Additional Information

Alternative name: Lyn

Immunogen: Bacterially expressed recombinant fragment of human Lyn (aa 8 - 238).

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: LYN-01

Description: Lyn is a Src-family protein tyrosine kinase that is predominantly expressed in hematopoieticcells. It is associated with a number of cell surface receptors including the B cell antigenreceptor (BCR) and Fc receptors. Upon their triggering, Lyn phosphorylates subunits ofthese receptors in a cholesterol-dependent manner, utilizing the plasma membrane lipid raftsystem. The phosphorylated intracellular domains of the receptors are accessible forcytoplasmic Syk tyrosine kinase, which is activated by Lyn-mediated phosphorylation andwhich transduces the signal to downstream adaptors. Lyn is abnormally distributed in acutemyeloid leukemia cells and seems to be a novel pharmacologic target of this disease.

Specificity: The antibody LYN-01 reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinaseexpressed in hematopoietic tissues.

Application Details

Application Notes: Western Blotting - Recommended dilution: 1-2 µg/ml, 60 min Positive control: RBL rat basophilic leukemia cell lineJURKAT human peripheral blood T cell leukemia cell lineA-431 human epidermoid carcinoma cell lineU-937 human histiocytic lymphoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% laurylmaltoside in 20 mMTris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60min on ice. Centrifuge to remove cell debris. Mix lysate (1: 1) with non-reducing SDS-PAGEsample buffer. Application note: Non-reducing conditions. SDS-PAGE (12% separating gel).

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein A-affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Signaling, Tyrosine Kinases

Restrictions: For Research Use only

Images

Western

Publications

Publications: Queval, Nicolas, Beau: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." in: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, 2011 (PubMed).