Linker For Activation of T Cells Family, Member 2 (LAT2) antibody

Antigen: Linker For Activation of T Cells Family, Member 2 (LAT2)

Synonyms: LAB, NTAL, WSCR5, WBSCR5, HSPC046, WBSCR15, LAT2, MGC139435

Clonality: Monoclonal (NAP-07)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN94439

Additional Information

Alternative name: NTAL / LAB

Immunogen: Recombinant cytoplasmic domain (aa 91-243) of human NTAL.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: NAP-07

Description: NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NKcells, mast cells and macrophages. It is a negative regulator of early stages ofBCR-dependent B cell signaling and serves as a negative regulator also in mast cells.However, in mast cells, NTAL also contributes to some activation processes, partiallyoverlapping with LAT function.

Specificity: The antibody NAP-07 reacts with Non-T cell activation linker (NTAL), also known as LAB(linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present inmembrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.

Application Details

Application Notes: Flow Cytometry - Positive tissue: RAJI human lymphoma cell lineRAMOS human lymphoma cell line. - Recommended dilution: 2 µg/ml, incubation 60 min Positive control: RAMOS human lymphoma cell line. Negative control: HeLa human cervix carcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12% separating SDS-PAGE gel.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Confocal microscopy

Publications

Publications: Duque-Afonso, Yalcin, Berg et al.: "The HDAC class I-specific inhibitor entinostat (MS-275) effectively relieves epigenetic silencing of the LAT2 gene mediated by AML1/ETO." in: Oncogene, Vol. 30, Issue 27, pp. 3062-72, 2011 (PubMed).