LAT2 antibody (Cytoplasmic Domain)

Catalog No. ABIN94439

There are 5+ publications for this product available. The Mouse Monoclonal anti-LAT2 antibody is suitable to detect LAT2 in samples from Human and Mouse. It has been validated for WB, IP, ICC and ICFC.

Quick Overview for LAT2 antibody (Cytoplasmic Domain) (ABIN94439)

Target: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC)

Clone: NAP-07

Product Details anti-LAT2 Antibody

Binding Specificity: AA 91-243, Cytoplasmic Domain

Purpose: Anti-NTAL Purified

Cross-Reactivity (Details): Mouse, Human, Other not tested

Characteristics: The antibody NAP-07 reacts with an intracellular epitope of Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein associating with lipid raft components in the plasma membrane of B lymphocytes, NK cells and myeloid cells.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Recombinant cytoplasmic domain (aa 91-243) of human NTAL.

Isotype: IgG1

Application Details

Application Notes: Western blotting: Recommended dilution: 1-2 μg/mL.
Flow cytometry: Intracellular staining, recommended dilution: 1-4 μg/mL, positive tissue: RAJI human lymphoma cell line, RAMOS human lymphoma cell line.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-LAT2 antibody (ABIN94439)

Duque-Afonso, Yalcin, Berg, Abdelkarim, Heidenreich, Lübbert: "The HDAC class I-specific inhibitor entinostat (MS-275) effectively relieves epigenetic silencing of the LAT2 gene mediated by AML1/ETO." in: Oncogene, Vol. 30, Issue 27, pp. 3062-72, (2011) (PubMed).

Lebduska, Korb, T?mová, Heneberg, Dráber: "Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells." in: Journal of immunological methods, Vol. 328, Issue 1-2, pp. 139-51, (2007) (PubMed).

Tkaczyk, Horejsi, Iwaki, Draber, Samelson, Satterthwaite, Nahm, Metcalfe, Gilfillan: "NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation." in: Blood, Vol. 104, Issue 1, pp. 207-14, (2004) (PubMed).

Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, (2004) (PubMed).

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).

Target Details for LAT2

Target: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Alternative Name: NTAL

Background: Linker for activation of T cells family member 2,NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.,LAB, LAT2

Gene ID: 56743

UniProt: Q9JHL0

Pathways: Fc-epsilon Receptor Signaling Pathway, BCR Signaling