Phosphotyrosine antibody

Catalog No. ABIN94452

Cited in 3+ publications. The Mouse Monoclonal anti-Phosphotyrosine antibody (Clone P-Tyr-01) (ABIN94452) specifically detects Phosphotyrosine in WB, FACS and ICC. The antibody is reactive with Various Species samples.

Quick Overview for Phosphotyrosine antibody (ABIN94452)

Target: Phosphotyrosine

Reactivity: Various Species

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: P-Tyr-01

Product Details anti-Phosphotyrosine Antibody

Purpose: Anti-Phosphotyrosine Purified

Cross-Reactivity (Details): Broad

Characteristics: The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Phosphotyrosine conjugated to bovine serum albumin.

Isotype: IgG1

Application Details

Application Notes: Western blotting: Recommended dilution: 1-2 μg/mL. Flow cytometry: Recommended dilution: 2-5 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Phosphotyrosine antibody (ABIN94452)

Biebl, Riedl, Buchner: "Hsp90 Co-chaperones Form Plastic Genetic Networks Adapted to Client Maturation." in: Cell reports, Vol. 32, Issue 8, pp. 108063, (2020) (PubMed).

Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, (2007) (PubMed).

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Target Type: Amino Acid

Background: PY

Gene ID: 35