Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) antibody

Antigen: Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2)

Synonyms: SLP76, SLP-76

Clonality: Monoclonal (SLP-76-03)

Host: Mouse

Reactivity: Human, Pig (Porcine), Mouse (Murine)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN94466

Additional Information

Alternative name: SLP76

Immunogen: Bacterially expressed fusion protein representing amino acids 216-434 of human SLP76with histidine tag

Format: Purified

Isotype: IgG2b  (Matching secondary antibodies)

Clone: SLP-76-03

Description: SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor proteinwhich translocates to the plasma mambrane and is involved in multiple signaling pathwaysin T cells, mast cells, neutrophils and platelets, B cells express its analog SLP65/BLNK (Bcell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinasesand couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2,SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. TheSH2 domain of SLP76 has been identified as the region involved in binding theserine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator bypromoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting thepathway leading to AP-1 activation.

Specificity: The polyclonal antibody reacts with SLP76, a 76kDa cytosolic adaptor protein that isinvolved in signaling of various hematopoietic cells, such as T cells, mast cells orneutrophils, in B cells, however, it is replaced by SLP65.

Application Details

Application Notes: Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml. Positive tissue: thymus

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-G affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Signaling

Restrictions: For Research Use only

Images

Western Blotting analysis (reducing conditions) of whole cell lysate using anti-SLP76 (SLP-76/03).Lane 1: HPB-ALL human peripheral blood T cell leukemia cell lineLane 2: JURKAT human peripheral blood T cell leukemia cell lineLane 3: RAMOS human Burkitt lymphoma cell line