Lin-28 Homolog A (C. Elegans) (LIN28A) (AA 115-147), (Middle Region) antibody

Details for Product No. ABIN953174
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Antigen
Synonyms Lin28, csdd1, lin-28, lin28, zcchc1, LIN28, CSDD1, LIN-28, ZCCHC1, lin-28A, AL024421, ENSMUSG00000070700, Gm10299, Lin-28, Tex17, RGD1566408, sb:cb650, zgc:55584
Epitope
AA 115-147, Middle Region
(12), (8), (8), (6), (5), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(125), (34), (27), (1), (1), (1)
Host
Rabbit
(90), (41)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(100), (50), (41), (36), (22), (17), (17), (14), (10), (10), (6), (5), (3), (1)
Pubmed 5 references available
Quantity 0.4 mL
Shipping to United States ( )
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Immunogen KLH conjugated synthetic peptide between 115-147 amino acids from the Central region of human LIN28A
Isotype Ig
Specificity This antibody recognizes Human and Mouse LIN28A (Center).
Purification Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Alternative Name LIN28 (LIN28A Antibody Abstract)
Background Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.Synonyms: CSDD1, LIN-28, LIN28A, Lin-28 homolog A, ZCCHC1
Molecular Weight 22743 Da
Gene ID 79727
NCBI Accession NP_078950
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Peng, Maihle, Huang: "Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer." in: Oncogene, Vol. 29, Issue 14, pp. 2153-9, 2010 (PubMed).

Qiu, Ma, Wang et al.: "Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells." in: Nucleic acids research, Vol. 38, Issue 4, pp. 1240-8, 2010 (PubMed).

Iliopoulos, Hirsch, Struhl: "An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links inflammation to cell transformation." in: Cell, Vol. 139, Issue 4, pp. 693-706, 2009 (PubMed).

Heo, Joo, Kim et al.: "TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation." in: Cell, Vol. 138, Issue 4, pp. 696-708, 2009 (PubMed).

West, Viswanathan, Yabuuchi et al.: "A role for Lin28 in primordial germ-cell development and germ-cell malignancy." in: Nature, Vol. 460, Issue 7257, pp. 909-13, 2009 (PubMed).

Validation Images
Catalog No. ABIN953174
357.50 $
Plus shipping costs $45.00
Quantity
Price
0.4 mL
357.50 $

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