PF4 antibody (Biotin)

Catalog No. ABIN954176

The Mouse Monoclonal anti-PF4 antibody has been validated for WB and EIA. It is suitable to detect PF4 in samples from Human.

Quick Overview for PF4 antibody (Biotin) (ABIN954176)

Target: PF4 (Platelet Factor 4 (PF4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PF4 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: KKO

Product Details anti-PF4 Antibody (Biotin)

Specificity: This antibody is specific for Human Platelet Factor IV/heparin complexes. AM31851BT-N (clone KKO), unlike AM31850BT-N (clone RTO) which binds PF4 alone, is specific for PF4/heparin complexes and is seen to have similar binding properties as auto-antibodies found in HIT/HITT.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Immunogen: Human Factor IV purified from the supernatant of Thrombin activated platelets.

Isotype: IgG2b

Application Details

Application Notes: ELISA: Use at 0.08 μg/mL following coating plate with PF4/ heparin complex. This can be done by incubating PF4 with an excess of heparin (100-200 U/mL), while coating the plate.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PF4

Target: PF4 (Platelet Factor 4 (PF4))

Alternative Name: Platelet Factor 4 / PF4

Background: Platelet Factor IV (PF4) is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity. Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT) is a life-threatening complication that manifests itself in a small population of patients exposed to intravenous-heparin. It is characterized by the production of PF4/heparin auto-antibodies. These auto-antibodies bind the PF4/heparin complexes and subsequently bind the FCGIIa receptor on the platelets surface through their Fc region. This activates the platelets and can initiate clot formation.Synonyms: C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4

Gene ID: 5196

NCBI Accession: NP_002610

UniProt: P02776