Biotin antibody
| Antigen |
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| Protein Type |
Native |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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FITC (28), HRP (23), Texas Red (TR) (20), Alkaline Phosphatase (AP) (16), TRITC (7), RPE (6), Rhodamine (6), PE (4), Biotin (3), DyLight 550 (2), DyLight 594 (2), DyLight 633 (2), DyLight 650 (2), 488-NHS Ester (1), Cy2 (1), Cy5 (1), DTAF (1), DyLight 350 (1), DyLight 800 (1)
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Application
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 Alternatives ELISA, Western Blotting (WB), Immunoprecipitation (IP)
ELISA (112), Western Blotting (WB) (93), Flow Cytometry (FACS) (75), Immunohistochemistry (IHC) (68), Immunofluorescence (IF) (49), Immunoprecipitation (IP) (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Dot Blot (Dot) (6), Fluorescence Microscopy (FM) (6), Immunodiffusion (ID) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunomicroscopy (IM) (2), Immunoelectrophoresis (IEP) (1)
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| Catalog no. |
ABIN95626 |
| Quantity |
2 mL (78 mg/mL) (Variants) |
| Price |
214.50 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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Immunogen
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Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
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Format
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Lyophilized
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Reconstitution
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Restore with deionized water (or equivalent)
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Characteristics
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Concentration Definition: by Refractometry
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Application Notes
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Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.
ELISA Dilution: 1:100,000
Western Blot Dilution: 1:10,000 - 1:25,000
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Concentration
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78 mg/mL
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Purification
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Antiserum
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Buffer
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0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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Preservative
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0.01% (w/v) Sodium azide
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Storage
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Expiration date is one (1) year from date of opening. None. Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Research Area
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Signaling, Metabolism, Epitope Tags, Tags/Labels
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Restrictions
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For Research Use only
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Titus JA, Haugland R, Sharrow SO et al.: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: J Immunol Methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).
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Alternatives for antigen "Biotin", type "Antibodies"
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Hosts
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Goat (126), Rabbit (47), Mouse (30), Sheep (1)
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Reactivities
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All species (18), Human (3)
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Applications
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ELISA (112), Western Blotting (WB) (93), Flow Cytometry (FACS) (75), Immunohistochemistry (IHC) (68), Immunofluorescence (IF) (49), Immunoprecipitation (IP) (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Dot Blot (Dot) (6), Fluorescence Microscopy (FM) (6), Immunodiffusion (ID) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunomicroscopy (IM) (2), Immunoelectrophoresis (IEP) (1)
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Conjugates
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FITC (28), HRP (23), Texas Red (TR) (20), Alkaline Phosphatase (AP) (16), TRITC (7), RPE (6), Rhodamine (6), PE (4), Biotin (3), DyLight 550 (2), DyLight 594 (2), DyLight 633 (2), DyLight 650 (2), 488-NHS Ester (1), Cy2 (1), Cy5 (1), DTAF (1), DyLight 350 (1), DyLight 800 (1)
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2013 BioMed Central Research Awards
