Carboxypeptidase Y (CPY) antibody

Antigen: Carboxypeptidase Y (CPY)

Clonality: Polyclonal

Host: Rabbit

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN96043

Additional Information

Alternative name: Carboxypeptidase Y

Immunogen: Carboxypeptidase Y [Bakers Yeast]

Format: Lyophilized

Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Carboxypeptidase Y [Bakers Yeast]. Cross reactivity against Carboxypeptidase Y from other tissues and species may occur but have not been specifically determined.

Application Details

Application Notes: Recommended Dilutions: This product has been assayed against 1.0 ug of Carboxypeptidase Y [Bakers Yeast] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code ABIN101990 and (ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

Concentration: 90.0 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.

Research Area: Proteolysis / Ubiquitin, Metabolism, Amino Acids, Enzymes

Restrictions: For Research Use only

Images

Both the antiserum and IgG fractions of anti-Carboxypeptidase Y (Baker's Yeast) are shown to detect under reducing conditions of SDS-PAGE the 61,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (ABIN101990) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.

Publications

Publications: von Numers, Survila, Aalto et al.: "Requirement of a Homolog of Glucosidase II {beta}-Subunit for EFR-Mediated Defense Signaling in Arabidopsis thaliana." in: Molecular plant, 2010 (PubMed).

Martinez Benitez, Stolz, Becher et al.: "Mnl2, a novel component of the ER associated protein degradation pathway." in: Biochemical and biophysical research communications, 2011 (PubMed). Method employed by authors: Immunoprecipitation (IP) Further details: 900 ul of the supernatant was transferred to a microtube containing 3 ul of polyclonal CPY antibody for immunoprecipitation