CPY antibody (Biotin)

Catalog No. ABIN1607713

Product Details anti-CPY Antibody (Biotin)

Target: CPY (Carboxypeptidase Y (CPY))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPY antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Carboxypeptidase Y from other sources may occur but has not been specifically determined.

Purity: Anti-Carboxypeptidase Y is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y [Baker’s Yeast].

Endotoxin Level: Low Endotoxin : No

Immunogen: Carboxypeptidase Y [Baker’s Yeast]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Carboxypeptidase Y has been assayed against 1.0 µg of Carboxypeptidase Y in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for CPY

Target: CPY (Carboxypeptidase Y (CPY))

Alternative Name: Carboxypeptidase Y (CPY Products)

Synonyms: AO090103000332 antibody, carboxypeptidase Y antibody, CC1G_12159 antibody, PAS_chr1-4_0013 antibody, MCYG_00135 antibody, MGYG_00701 antibody, AFUA_5G14610 antibody, ANI_1_238044 antibody, AOR_1_542034 antibody

Background: Anti-Carboxypeptidase Y antibody is a participant in degrading small peptides. Carboyxpeptidase Y preferentially digests peptides containing aliphatic or hydrophobic residue in P1’ position, as well as methionine, leucine, or phenylalanine in P1 position of ester substrate. A member of the peptidase S10 family, Anti-Carboxypeptidase Y is ideal for researchers interested in Metabolism, Signal Transduction, and Cell Biology research. Synonyms: Carboxypeptidase Y antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody

Gene ID: 855343

UniProt: P00729