Catalase (CAT) antibody

Antigen: Catalase (CAT)

Synonyms: MGC138422, MGC138424, Cas1, Cs-1, Cas-1, 2210418N07, CS1, RATCATL, RATCAT01, fb68a12, wu:fb68a12, CAT, CATA, CT21282, DMCATHPO, DROCATHPO, U00145, bs36h11.y1, DmelCG6871, CG6871, MGC128112, CATALASE, CATALASE 2, T12J5.2, Cat, GB11648, catalase, MGC147040, DKFZp469E0232

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Catalog no.: ABIN96131

Additional Information

Alternative name: Catalase

Immunogen: Catalase [Bovine Liver]

Format: Lyophilized

Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Catalase [Bovine Liver]. Cross reactivity against Catalase from other tissues and species may occur but have not been specifically determined.

Synonyms: MGC138422, MGC138424, Cas1, Cs-1, Cas-1, 2210418N07, CS1, RATCATL, RATCAT01, fb68a12, wu:fb68a12, CAT, CATA, CT21282, DMCATHPO, DROCATHPO, U00145, bs36h11.y1, DmelCG6871, CG6871, MGC128112, CATALASE, CATALASE 2, T12J5.2, Cat, GB11648, catalase, MGC147040, DKFZp469E0232

Application Details

Application Notes: All assays should be optimized by the end user. Recommended dilutions may be listed below: ELISA 1:100000-1:1000000 WB 1:5000-1:20000 IHC Optimal titers for this applications should be determined by the researcher.

Concentration: 85.0 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent).Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeksat 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at-20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.

Research Area: Cancer, Organelles, Heat Shock Proteins, Cell/Tissue Markers

Restrictions: For Research Use only

Images

D3cpv-SKL co-localizes after cell membrane permeabilization with the peroxisome protein markers, catalase and PMP70. HeLa cells transiently expressing D3cpv-SKL were permeabilized with digitonin (A and D). Cells were then fixed and treated with an antibody against a peroxisome marker, catalase (B) or PMP70 (E) and then with a secondary antibody conjugated to Alexa 568. Co-localization of the two signals is shown in C and F. Scale bar, 10 μm. (Source: Drago et al. (2008). J Biol Chem.Vol. 283(21):14384-90)

Publications

Publications: Shashoua, Adams, Volodina et al.: "New synthetic peptides can enhance gene expression of key antioxidant defense enzymes in vitro and in vivo." in: Brain research, Vol. 1024, Issue 1-2, pp. 34-43, 2004 (PubMed).

Drago, Giacomello, Pizzo et al.: "Calcium dynamics in the peroxisomal lumen of living cells." in: The Journal of biological chemistry, Vol. 283, Issue 21, pp. 14384-90, 2008 (PubMed).

Vincent, Kato, McLean et al.: "Sensory neurons and schwann cells respond to oxidative stress by increasing antioxidant defense mechanisms." in: Antioxidants & redox signaling, Vol. 11, Issue 3, pp. 425-38, 2009 (PubMed).

Rosicarelli, Stefanini: "DEHP effects on histology and cell proliferation in lung of newborn rats." in: Histochemistry and cell biology, Vol. 131, Issue 4, pp. 491-500, 2009 (PubMed).

Miyata, Hosoi, Mukai et al.: "In vitro import of peroxisome-targeting signal type 2 (PTS2) receptor Pex7p into peroxisomes." in: Biochimica et biophysica acta, Vol. 1793, Issue 5, pp. 860-70, 2009 (PubMed).

Apraiz, Cajaraville, Cristobal: "Peroxisomal proteomics: biomonitoring in mussels after the Prestige's oil spill." in: Marine pollution bulletin, Vol. 58, Issue 12, pp. 1815-26, 2009 (PubMed).

Krysko, Stevens, Langenberg et al.: "Peroxisomes in zebrafish: distribution pattern and knockdown studies." in: Histochemistry and cell biology, Vol. 134, Issue 1, pp. 39-51, 2010 (PubMed).