Acetylcholinesterase antibody (N-Term)

Catalog No. ABIN965502

Product Details anti-Acetylcholinesterase Antibody

Target: Acetylcholinesterase (AChE)

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetylcholinesterase antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Purification: Purified by antigen-specific affinity chromatography.

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to N-terminal residues of human ACHE (N-terminal extended acetylcholinesterase)

Application Details

Application Notes: ELISA, Western blotting: 1µg/ml for 2hrs.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: This antibody is stored in PBS, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-Acetylcholinesterase antibody (ABIN965502)

Meshorer, Toiber, Zurel, Sahly, Dori, Cagnano, Schreiber, Grisaru, Tronche, Soreq: "Combinatorial complexity of 5' alternative acetylcholinesterase transcripts and protein products." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 29740-51, (2004) (PubMed).

Velan, Grosfeld, Kronman, Leitner, Gozes, Lazar, Flashner, Marcus, Cohen, Shafferman: "The effect of elimination of intersubunit disulfide bonds on the activity, assembly, and secretion of recombinant human acetylcholinesterase. Expression of acetylcholinesterase Cys-580----Ala mutant." in: The Journal of biological chemistry, Vol. 266, Issue 35, pp. 23977-84, (1992) (PubMed).

Shafferman, Kronman, Flashner, Leitner, Grosfeld, Ordentlich, Gozes, Cohen, Ariel, Barak: "Mutagenesis of human acetylcholinesterase. Identification of residues involved in catalytic activity and in polypeptide folding." in: The Journal of biological chemistry, Vol. 267, Issue 25, pp. 17640-8, (1992) (PubMed).

Soreq, Ben-Aziz, Prody, Seidman, Gnatt, Neville, Lieman-Hurwitz, Lev-Lehman, Ginzberg, Lipidot-Lifson: "Molecular cloning and construction of the coding region for human acetylcholinesterase reveals a G + C-rich attenuating structure." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 24, pp. 9688-92, (1991) (PubMed).

Target Details for Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Alternative Name: ACHE (AChE Products)

Synonyms: ACEE antibody, ARACHE antibody, N-ACHE antibody, YT antibody, Ache antibody, GB14873 antibody, zgc:92550 antibody, ACE antibody, Dsim\\GD20515 antibody, GD20515 antibody, dsim_GLEANR_4292 antibody, ache antibody, mE1a antibody, mE1b antibody, mE1c antibody, mE1c-long antibody, mE1d antibody, mE1d' antibody, mE1e antibody, arache antibody, n-ache antibody, acetylcholinesterase (Cartwright blood group) antibody, acetylcholinesterase 2 antibody, acetylcholinesterase antibody, Acetylcholine esterase antibody, acetylcholinesterase (Cartwright blood group) L homeolog antibody, collagen type I alpha 2 chain antibody, ACHE antibody, AChE-2 antibody, Ache antibody, ache antibody, Dsim\Ace antibody, ache.L antibody, COL1A2 antibody, ACE-1 antibody

Background: ACHE(acetylcholinesterase) rapidly hydrolyzes choline released into the synapse. ACHE interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers. ACHE is responsible for the Yt blood group system. The molecular basis of the Yt(a)=Yt1/ Yt(b)=Yt2 blood group antigens is a single variation in position 353, His-353 corresponds to Yt(a) and the rare variant with Asn-353 to Yt(b).

Pathways: Skeletal Muscle Fiber Development