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Acetylcholinesterase antibody (C-Term)

Verified AChE Reactivity: Human WB, ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN185357
  • Target See all Acetylcholinesterase (AChE) Antibodies
    Acetylcholinesterase (AChE)
    Binding Specificity
    • 15
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 49
    • 34
    • 33
    • 18
    • 9
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 90
    • 8
    • 7
    • 1
    Goat
    Clonality
    • 99
    • 7
    Polyclonal
    Conjugate
    • 53
    • 22
    • 8
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Acetylcholinesterase antibody is un-conjugated
    Application
    • 85
    • 47
    • 22
    • 16
    • 13
    • 13
    • 11
    • 8
    • 8
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    ACHE
    Sequence
    QFDHYSKQDR CSDL
    Specificity
    This antibody is expected to recognise isoform NP_000656.1 only (the ubiquitously expressed, hydrophillic form).
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence QFDHYSKQDRCSDL, from the C Terminus of the protein sequence according to NP_000656.1.
    Isotype
    IgG
  • Application Notes

    Western Blot: Approx 70 kDa band observed in HepG2 and Jurkat cell lysates (calculated MW of 67.8 kDa according to NP_000656.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm, plasma membrane and nucleus of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recomm

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Acetylcholinesterase (AChE)
    Alternative Name
    ACHE (AChE Products)
    Synonyms
    ACEE antibody, ARACHE antibody, N-ACHE antibody, YT antibody, Ache antibody, GB14873 antibody, zgc:92550 antibody, ACE antibody, Dsim\\GD20515 antibody, GD20515 antibody, dsim_GLEANR_4292 antibody, ache antibody, mE1a antibody, mE1b antibody, mE1c antibody, mE1c-long antibody, mE1d antibody, mE1d' antibody, mE1e antibody, arache antibody, n-ache antibody, acetylcholinesterase (Cartwright blood group) antibody, acetylcholinesterase 2 antibody, acetylcholinesterase antibody, Acetylcholine esterase antibody, acetylcholinesterase (Cartwright blood group) L homeolog antibody, collagen type I alpha 2 chain antibody, ACHE antibody, AChE-2 antibody, Ache antibody, ache antibody, Dsim\Ace antibody, ache.L antibody, COL1A2 antibody, ACE-1 antibody
    Background
    ACHE, YT, acetylcholinesterase (YT blood group), ARACHE, N-ACHE, OTTHUMP00000211347, OTTHUMP00000211349, OTTHUMP00000211356, acetylcholinesterase, apoptosis-related acetylcholinesterase
    Gene ID
    43
    NCBI Accession
    NP_000656, NP_001269378
    Pathways
    Skeletal Muscle Fiber Development
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