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BRAF antibody

BRAF Reactivity: Human WB, ELISA, IHC Host: Mouse Monoclonal 1H12F1-1H12G10-1F12F11C9 unconjugated
Catalog No. ABIN965693
  • Target See all BRAF Antibodies
    BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
    Reactivity
    • 195
    • 65
    • 46
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 174
    • 21
    • 2
    Mouse
    Clonality
    • 162
    • 35
    Monoclonal
    Conjugate
    • 117
    • 13
    • 12
    • 10
    • 8
    • 8
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This BRAF antibody is un-conjugated
    Application
    • 111
    • 110
    • 76
    • 31
    • 25
    • 18
    • 13
    • 13
    • 8
    • 7
    • 6
    • 3
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Purification
    Ascitic fluid
    Immunogen
    Purified recombinant fragment of BRAF expressed in E. Coli.
    Clone
    1H12F1-1H12G10-1F12F11C9
    Isotype
    IgG1
    Top Product
    Discover our top product BRAF Primary Antibody
  • Application Notes
    Western Bloting: 1/500 - 1/2000.
    Immunohistochemistry: 1/200 - 1/1000.
    ELISA: Propose dilution 1/10000.
    Not yet tested in other applications.
    Determining optimal working dilutions by titration test.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Kim, Giuliano, Turner, Gaffney, Umetani, Kitago, Elashoff, Hoon: "Lymphatic mapping establishes the role of BRAF gene mutation in papillary thyroid carcinoma." in: Annals of surgery, Vol. 244, Issue 5, pp. 799-804, (2006) (PubMed).

    Terai, Matsuda: "The amino-terminal B-Raf-specific region mediates calcium-dependent homo- and hetero-dimerization of Raf." in: The EMBO journal, Vol. 25, Issue 15, pp. 3556-64, (2006) (PubMed).

    Noda, Kato, Yoshikawa, Arai, Togashi, Nagai, Konishi, Miki: "Frequent involvement of ras-signalling pathways in both polypoid-type and flat-type early-stage colorectal cancers." in: Journal of experimental & clinical cancer research : CR, Vol. 25, Issue 2, pp. 235-42, (2006) (PubMed).

  • Target
    BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
    Alternative Name
    BRAF (BRAF Products)
    Background
    BRAF(V-raf murine sarcoma viral oncogene homolog B1 ) is the main effectors recruited by GTP-bound Ras to activate the MEKMAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439).B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK),it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers.The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi,primary cutaneous melanomas and colorectal cancers.
    Synonyms: BRAF
    Gene ID
    673
    Pathways
    MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
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