BRAF antibody

Catalog No. ABIN1496954

The Mouse Monoclonal anti-BRAF antibody is suitable to detect BRAF in samples from Human, Rat, Monkey and Dog. It has been validated for WB, IHC, IF and IHC (p).

Quick Overview for BRAF antibody (ABIN1496954)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human, Rat, Monkey, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3A7

Product Details anti-BRAF Antibody

Specificity: Human BRAF / B-Raf

Purification: Protein A/G purified

Immunogen: Protein expressed in 293T cell transfected with human BRAF expression vector.

Type of Immunogen: Transfected Cells

Isotype: IgG2a

Application Details

Application Notes: Approved: IF (1:100), IHC, IHC-P (1:50), WB (1:2000)

Usage: Concentration: 0.5-1.0 mg/mL (Lot dependent)

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.3, 1 % BSA, 50 % glycerol, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF / B-Raf

Background: Name/Gene ID: BRAF
Subfamily: Raf
Family: Protein Kinase

Synonyms: BRAF, 94 kDa B-raf protein, B-raf, BRAF1, B Raf, Proto-oncogene B-Raf, p94, B-RAF1, NS7, ONCOGENE BRAF, RAFB1

Gene ID: 673

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy