Cytokeratin 5/6/18 (45 & 56.5kDa) antibody

Antigen: Cytokeratin 5/6/18 (45 & 56.5kDa)

Clonality: Monoclonal (LP34)

Host: Mouse

Reactivity: Human, Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN965963

Additional Information

Immunogen: BALB/C mice were immunized with human psoriatic epidermis.

Isotype: IgG1

Clone: LP34

Description: The K059 is supplied in two screw cap microtubes, labeled A and B. Tube A, which has an orange cap, contains 0.5 mL of dehydrated antibody reconstitution buffer. Tube B, which has a blue cap, contains the dehydrated concentrated monoclonal antibody. The light green color is the combination of No. 1 blue and No. 5 yellow food dyes, it serves as an indicator for the reconstitution procedure.

Specificity: This antibody reacts with cytokeratin 5, 6 and 18 (58, 56 and 45 kD) polypeptides.

Application Details

Application Notes: Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:15 to 1:20 in IHC. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique) is strongly recommended for consistent and reproducible results. This product can also be used on frozen tissue sections. The optimal conditions should be determined by the individual laboratory.

Purification: Dehydrated.

Storage: Refrigerate at 4°C. Do not freeze.

Restrictions: For Research Use only

Publications

Product: Jarasch, Nagle, Kaufmann et al.: "Differential diagnosis of benign epithelial proliferations and carcinomas of the breast using antibodies to cytokeratins." in: Human pathology, Vol. 19, Issue 3, pp. 276-89, 1988 (PubMed).

Balaton, Nehama-Sibony, Gotheil et al.: "Distinction between hepatocellular carcinoma, cholangiocarcinoma, and metastatic carcinoma based on immunohistochemical staining for carcinoembryonic antigen and for cytokeratin 19 on paraffin sections." in: The Journal of pathology, Vol. 156, Issue 4, pp. 305-10, 1989 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).