Keratin 5,6,18 antibody

Catalog No. ABIN6937075

Product Details

Target: Keratin 5,6,18

Reactivity: Human, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Specificity: It recognizes polypeptides of 58 kDa, 56 kDa, and 45 kDa, identified as cytokeratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. This MAb shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This MAb is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.

Purification: Purified by Protein A/G

Immunogen: Detergent-insoluble fraction of psoriatic human epidermis

Clone: SPM267

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa, HepG2 or PC12 cells. Tonsil or Skin.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details

Target: Keratin 5,6,18

Alternative Name: Cytokeratin 5/6/18

Molecular Weight: 45-58kDa